PerCP anti-mouse NK-1.1 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1484	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

NK-1⁺ cells from mouse spleen and bone marrow

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with PerCP under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm.

Excitation Laser

Blue Laser (488 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation^1,2, complement-dependent cytotoxicity³, in vivo depletion^4,5,9,10, mediation of in vitro redirected lysis⁶, blocking of NK cell function⁷, induction of proliferation⁸, immunohistochemical staining of frozen sections¹¹, immunofluorescence microscopy¹¹, and spatial biology (IBEX)^16,17. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 108712).

Application References (PubMed link indicates BioLegend citation)

Carlyle JR, et al. 1999. J. Immunol. 162:5917. (IP)
Sentman CL, et al. 1989. Hybridoma 8:605. (IP)
Koo GC, et al. 1984. Hybridoma 3:301. (Cyt)
Sentman CL, et al. 1989. J. Immunol. 142:1847. (Deplete)
Koo GC, et al. 1986. J. Immunol. 137:3742. (Deplete)
Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
Kung SK, et al. 1999. J. Immunol. 162:5876. (Block)
Reichlin A, et al. 1998. Immunol. Cell Biol. 76:143.
Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (Deplete)
Kanwar JR, et al. 2001. J. Natl. Cancer Inst. 93:1541. (IHC, IF)
Kroemer A, et al. 2008. J. Immunol. 180:7818. PubMed
Kim JY, et al. 2009. Exp Mol Med. 30:288. PubMed
Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
Lee H, et al. 2014. Invest Ophthalmol Vis Sci. 55:2885. PubMed
Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed

Product Citations

Hsieh C, et al. 2014. J Immunol. 193:3693. PubMed
Xu X, et al. 2018. Cell. 175:1336. PubMed
Liu S, et al. 2020. Cell Host & Microbe. 26(6):779-794.e8.. PubMed

RRID

AB_893602 (BioLegend Cat. No. 108725) AB_2132707 (BioLegend Cat. No. 108726)

Antigen Details

Structure: NKR-P1 gene family
Distribution: NK and NK-T cells in the NK1.1 mouse strains (C57BL, FVB/N, NZB)
Function: NK cell activation, IFN-γ production, cytotoxic granule release
Cell Type: NK cells, NKT cells
Biology Area: Immunology, Innate Immunity
Antigen References: 1. Lanier LL. 1997. Immunity 6:371.
2. Yokoyama WM, et al. 1993. Ann. Rev. Immunol. 11:613.
3. Koo GC, et al. 1986. J. Immunol. 137:3742.
4. Giorda R, et al. 1991. J. Immunol. 147:1701.
Gene ID: 17059 View all products for this Gene ID
UniProt: View information about NK-1.1 on UniProt.org

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