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首页 > 抗体蛋白 > 抗体
Pacific Blue™ anti-mouse NK-1.1 Antibody
产品名称：
Pacific Blue™ anti-mouse NK-1.1 Antibody
产品类别：
抗体
产品编号：
108721
产品应用：
108721
[价格]
规格 价格 库存
25µg ￥ 1414 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
NK-1+ cells from mouse spleen and bone marrow
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue? under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 1.0 ?g per 106 cells in 100 ?l volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, complement-dependent cytotoxicity3, in vivo depletion4,5,9,10, mediation of in vitro redirected lysis6, blocking of NK cell function7, induction of proliferation8, immunohistochemical staining of frozen sections11, immunofluorescence microscopy11, and spatial biology (IBEX)16,17. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 108712).

Application References

(PubMed link indicates BioLegend citation)
  1. Carlyle JR, et al. 1999. J. Immunol. 162:5917. (IP)
  2. Sentman CL, et al. 1989. Hybridoma 8:605. (IP)
  3. Koo GC, et al. 1984. Hybridoma 3:301. (Cyt)
  4. Sentman CL, et al. 1989. J. Immunol. 142:1847. (Deplete)
  5. Koo GC, et al. 1986. J. Immunol. 137:3742. (Deplete)
  6. Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
  7. Kung SK, et al. 1999. J. Immunol. 162:5876. (Block)
  8. Reichlin A, et al. 1998. Immunol. Cell Biol. 76:143.
  9. Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
  10. Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (Deplete)
  11. Kanwar JR, et al. 2001. J. Natl. Cancer Inst. 93:1541. (IHC, IF)
  12. Kroemer A, et al. 2008. J. Immunol. 180:7818. PubMed
  13. Kim JY, et al. 2009. Exp Mol Med. 30:288. PubMed
  14. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  15. Lee H, et al. 2014. Invest Ophthalmol Vis Sci. 55:2885. PubMed
  16. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
  17. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
Product Citations
  1. Christian D, et al. 2014. Infect Immun . 82:4056. PubMed
  2. Schneider C, et al. 2018. Cell. 174:271. PubMed
  3. B?iers C, et al. 2010. Blood. 115:5061. PubMed
  4. Molofsky A, et al. 2013. J Exp Med. 210:535. PubMed
  5. Mikami H, et al. 2020. Nat Commun. 11:1162. PubMed
  6. Wagage S, et al. 2014. J Immunol. 192:1661. PubMed
  7. Woolard M, et al. 2008. Infect Immun. 76:2651. PubMed
  8. Chun E, et al. 2020. Immunity. 51(5):871-884.e6.. PubMed
  9. Di Genua C, et al. 2020. Cancer Cell. 37(5):690-704.e8.. PubMed
  10. Booth CAG, et al. 2018. Cancer Cell. 33:274. PubMed
  11. Ni J, et al. 2020. Immunity. 52(6):1075-1087.e8. PubMed
  12. Leblanc P, et al. 2012. PLoS One. 7:e30872. PubMed
  13. Ricci B, et al. 2020. eLife. 9:e54659.. PubMed
  14. Dahlgren MW et al. 2019. Immunity. 50(3):707-722 . PubMed
  15. Di Genua C, et al. 2019. Haematologica. 104:2215. PubMed
  16. Tuong ZK, et al. 2021. Cell Rep. 37:110132. PubMed
  17. O'Leary CE, et al. 2021. Curr Protoc. 1:e77. PubMed
  18. Silver J, et al. 2011. J Immunol. 187:350. PubMed
  19. S?wen P et al. 2018. eLife. 7 pii: e41258. PubMed
  20. Verma M, et al. 2021. J Exp Med. 218:. PubMed
RRID
AB_2234352 (BioLegend Cat. No. 108721) AB_2132712 (BioLegend Cat. No. 108722)

Antigen Details

Structure
NKR-P1 gene family
Distribution

NK and NK-T cells in the NK1.1 mouse strains (C57BL, FVB/N, NZB)

Function
NK cell activation, IFN-γ production, cytotoxic granule release
Cell Type
NK cells, NKT cells
Biology Area
Immunology, Innate Immunity
Antigen References

1. Lanier LL. 1997. Immunity 6:371.
2. Yokoyama WM, et al. 1993. Ann. Rev. Immunol. 11:613.
3. Koo GC, et al. 1986. J. Immunol. 137:3742.
4. Giorda R, et al. 1991. J. Immunol. 147:1701.

Gene ID
17059 View all products for this Gene ID
UniProt
View information about NK-1.1 on UniProt.org

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