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Brilliant Violet 650™ anti-mouse NK-1.1 Anti
产品名称：
Brilliant Violet 650™ anti-mouse NK-1.1 Anti
产品类别：
抗体
产品编号：
108735
产品应用：
108735
[价格]
规格 价格 库存
125µL ￥ 3318 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
NK-1+ cells from mouse spleen and bone marrow
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650? under optimal conditions.
Concentration
?g sizes: 0.2 mg/mL
?L sizes: lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the ?g size, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume. For flow cytometric staining using the ?l size, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.


Brilliant Violet 650? excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650? is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, complement-dependent cytotoxicity3, in vivo depletion4,5,9,10, mediation of in vitro redirected lysis6, blocking of NK cell function7, induction of proliferation8, immunohistochemical staining of frozen sections11, immunofluorescence microscopy11, and spatial biology (IBEX)16,17. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 108712).

Application References

(PubMed link indicates BioLegend citation)
  1. Carlyle JR, et al. 1999. J. Immunol. 162:5917. (IP)
  2. Sentman CL, et al. 1989. Hybridoma 8:605. (IP)
  3. Koo GC, et al. 1984. Hybridoma 3:301. (Cyt)
  4. Sentman CL, et al. 1989. J. Immunol. 142:1847. (Deplete)
  5. Koo GC, et al. 1986. J. Immunol. 137:3742. (Deplete)
  6. Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
  7. Kung SK, et al. 1999. J. Immunol. 162:5876. (Block)
  8. Reichlin A, et al. 1998. Immunol. Cell Biol. 76:143.
  9. Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
  10. Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (Deplete)
  11. Kanwar JR, et al. 2001. J. Natl. Cancer Inst. 93:1541. (IHC, IF)
  12. Kroemer A, et al. 2008. J. Immunol. 180:7818. PubMed
  13. Kim JY, et al. 2009. Exp Mol Med. 30:288. PubMed
  14. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  15. Lee H, et al. 2014. Invest Ophthalmol Vis Sci. 55:2885. PubMed
  16. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
  17. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
Product Citations
  1. Harsha Krovi S, et al. 2020. Nat Commun. 4.790277778. PubMed
  2. Marchelletta RR, et al. 2021. J Clin Invest. 131:. PubMed
  3. Wong Fok Lung T, et al. 2020. Nat Microbiol. 141:5. PubMed
  4. Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed
  5. Kim SS, et al. 2022. Cancers (Basel). 14:. PubMed
  6. Lechner AJ et al. 2017. Cell stem cell. 21(1):120-134 . PubMed
  7. Ellis G, et al. 2015. EMBO Rep. 16: 1203-1218. PubMed
  8. Coursey T, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.83. PubMed
  9. Grzelak A, et al. 2018. Int J Mol Sci. 19:. PubMed
  10. Parikh BA, et al. 2020. Cell Rep. 32:107969. PubMed
  11. McFarland AP, et al. 2021. Immunity. 54(6):1320-1337.e4. PubMed
  12. Acker KP, et al. 2019. iScience. 19:281. PubMed
  13. Sivori S, et al. 2020. Cell Reports. 16(5):430-441. PubMed
  14. Kuhn JA, et al. 2021. Elife. 10:. PubMed
  15. Covarrubias AJ, et al. 2020. Nat Metab. 1265:2. PubMed
  16. Coronel MM, et al. 2020. Sci Adv. 6:eaba5573. PubMed
  17. Lu Y, et al. 2020. Immunity. 52:782. PubMed
  18. Dahlgren MW et al. 2019. Immunity. 50(3):707-722 . PubMed
  19. Kovacs SB, et al. 2021. STAR Protoc. 2:100244. PubMed
  20. Victorino F, et al. 2021. eLife. 0.416666666666667. PubMed
  21. Dallari S, et al. 2021. Cell Host Microbe. 29(6):1014-1029.e8. PubMed
  22. Asadi Shahmirzadi A, et al. 2020. Cell Metabolism. 32(3):447-456.e6. PubMed
  23. Meunier I, et al. 2015. PLoS One. 10: 0138055. PubMed
  24. Schaftenaar FH, et al. 2019. Sci Rep. 9:17391. PubMed
  25. Molgora M, et al. 2020. Cell. 182:886. PubMed
  26. Hackstein CP, et al. 2022. Nat Commun. 13:7472. PubMed
  27. Xu Y, et al. 2014. Proc Natl Acad Sci U S A. 111:6371. PubMed
  28. Silva HM, et al. 2019. J Exp Med. 216:786. PubMed
  29. Nascimento DC, et al. 2021. Immunity. :. PubMed
RRID
AB_11147949 (BioLegend Cat. No. 108735) AB_2563159 (BioLegend Cat. No. 108736)

Antigen Details

Structure
NKR-P1 gene family
Distribution

NK and NK-T cells in the NK1.1 mouse strains (C57BL, FVB/N, NZB)

Function
NK cell activation, IFN-γ production, cytotoxic granule release
Cell Type
NK cells, NKT cells
Biology Area
Immunology, Innate Immunity
Antigen References

1. Lanier LL. 1997. Immunity 6:371.
2. Yokoyama WM, et al. 1993. Ann. Rev. Immunol. 11:613.
3. Koo GC, et al. 1986. J. Immunol. 137:3742.
4. Giorda R, et al. 1991. J. Immunol. 147:1701.

Gene ID
17059 View all products for this Gene ID
UniProt
View information about NK-1.1 on UniProt.org

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