Purified anti-CD141 (Thrombomodulin) Antibody (Pre
- 产品名称：
- Purified anti-CD141 (Thrombomodulin) Antibody (Pre
- 产品类别：
- 抗体
- 产品编号：
- 902102
- 产品应用：
- 902102
- Verified Reactivity
- Mammalian
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution & 0.03% Thimerosal.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
- Application
-
IHC-F -?Quality tested
WB, ELISA?-?Verified
- Recommended Usage
Each lot of this antibody is quality control tested by immunohistochemical staining.
The optimal working dilution should be determined for each specific assay condition.
? WB: 1 - 2 ?g/ml (1:500 - 1:1000)
? ELISA: 1:1,000
? IHC: 1:100
Tissue Sections: Frozen tissue sections
Incubation: 60 minutes at room temperature
- Application Notes
This antibody is effective in immunoblotting, immunohistochemistry (IHC) and ELISA.
*Predicted MW = 60 kD
Positive control tissue: human placenta
This antibody reacts with an epitope within the EGF 5 domain and inhibits thrombin binding.
- Application References
(PubMed link indicates BioLegend citation) -
- Nagashima M, et al. 1993. J Biol Chem. 268:2888.
- Parkinson JF, et al. 1992. Biochem Biophys Res Commun. 185:567
- Product Citations
-
- Gruber CN, et al. 2020. Cell. 183:982. PubMed
- RRID
- AB_2734669 (BioLegend Cat. No. 902102) AB_2565016 (BioLegend Cat. No. 902101)
- Structure
- Single chain, type I membrane glycoprotein, 75 kD
- Distribution
-
Macrophages, monocytes, subset of myeloid dendritic cells, vascular endothelial cells, keratinocytes
- Function
- After thrombin binding, CD141 activates protein C, which degrades clotting factors Va and VIIIa and reduces the amount of thrombin generated.
- Interaction
- Protein C, Thrombin-activatable fibrinolysis inhibitor (TAFI), Platelet factor 4 (PF4)
- Ligand/Receptor
- Thrombin
- Cell Type
- Dendritic cells, Endothelial cells, Macrophages, Monocytes
- Biology Area
- Cell Biology, Neuroinflammation, Neuroscience, Signal Transduction
- Gene ID
- 7056 View all products for this Gene ID
- UniProt
- View information about CD141 on UniProt.org
