Ultra-LEAF™ Purified anti-mouse CD25 Antibod-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 1540	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

IL-2-dependent cytolytic mouse T-cell clone B6.1

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
IHC-F, IP, Block, Depletion - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per million cells in 100 ?l volume or 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation^1,2, in vitro blocking of IL-2 binding to low- and high-affinity receptors^1-4, growth inhibition of IL-2-dependent T-cell lines^1-4, in vivo depletion of CD25⁺CD4⁺ Treg cells^5-8,10, and immunohistochemical staining of acetone-fixed frozen sections². PC61 antibody recognizes a different epitope than 3C7 antibody (Cat. No. 101902).?For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 102040) with endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered.?

Application References (PubMed link indicates BioLegend citation)

Lowenthal JW, et al. 1985. Nature 315:669. (IP, Block)
Ceredig R, et al. 1985. Nature 314:98. (IP, IHC, Block)
Lowenthal JW, et al. 1985. J. Immunol. 135:3988. (Block)
Moreau JL, et al. 1987. Eur. J. Immunol. 17:929. (Block)
Takahashi T, et al. 2000. J. Exp. Med. 192:303. (Deplete)
Onizuka S, et al. 1999. Cancer Res. 59:3128. (Deplete)
Lei TC, et al. 2005. Blood 105:4865. (Deplete)
Pasare C, et al. 2004. Immunity 21:733. (Deplete)
León-Ponte M, et al. 2007. Blood 109:3139.
Cao OW, et al. 2007. Blood doi:10.1182/blood-2007-02-073304. (Deplete)
Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719.
Liu F, et al. 2011. Arch Toxicol. 85:1383. PubMed
Anguela XM, et al. 2013. Diabetes. 62:551. PubMed

Product Citations

Handke J, et al. 2021. Front Cardiovasc Med. 8:682458. PubMed
Sugiyama E, et al. 2020. Sci Immunol. 5:00. PubMed
Sharma M, et al. 2020. Circ Res. 127:335. PubMed

RRID

AB_2813922 (BioLegend Cat. No. 102057) AB_11150394 (BioLegend Cat. No. 102040) AB_2813923 (BioLegend Cat. No. 102058) AB_2813924 (BioLegend Cat. No. 102059) AB_2813925 (BioLegend Cat. No. 102060) AB_2813926 (BioLegend Cat. No. 102061)