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Brilliant Violet 421™ anti-mouse CD25 Antibo
产品名称：
Brilliant Violet 421™ anti-mouse CD25 Antibo
产品类别：
抗体
产品编号：
102033
产品应用：
102033
[价格]
规格 价格 库存
125µL ￥ 2618 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
IL-2-dependent cytolytic mouse T-cell clone B6.1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421? under optimal conditions.
Concentration
?g sizes: 0.2 mg/mL
?L sizes: lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining using the ?g size, the suggested use of this reagent is ≤0.125 ?g per million cells in 100 ?l volume. For immunofluorescent staining using ?l sizes, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421? excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421? is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, in vitro blocking of IL-2 binding to low- and high-affinity receptors1-4, growth inhibition of IL-2-dependent T-cell lines1-4, in vivo depletion of CD25+CD4+ Treg cells5-8,10, and immunohistochemical staining of acetone-fixed frozen sections2. PC61 antibody recognizes a different epitope than 3C7 antibody (Cat. No. 101902).?For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 102040) with endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered.?

Application References

(PubMed link indicates BioLegend citation)
  1. Lowenthal JW, et al. 1985. Nature 315:669. (IP, Block)
  2. Ceredig R, et al. 1985. Nature 314:98. (IP, IHC, Block)
  3. Lowenthal JW, et al. 1985. J. Immunol. 135:3988. (Block)
  4. Moreau JL, et al. 1987. Eur. J. Immunol. 17:929. (Block)
  5. Takahashi T, et al. 2000. J. Exp. Med. 192:303. (Deplete)
  6. Onizuka S, et al. 1999. Cancer Res. 59:3128. (Deplete)
  7. Lei TC, et al. 2005. Blood 105:4865. (Deplete)
  8. Pasare C, et al. 2004. Immunity 21:733. (Deplete)
  9. León-Ponte M, et al. 2007. Blood 109:3139.
  10. Cao OW, et al. 2007. Blood doi:10.1182/blood-2007-02-073304. (Deplete)
  11. Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719.
  12. Liu F, et al. 2011. Arch Toxicol. 85:1383. PubMed
  13. Anguela XM, et al. 2013. Diabetes. 62:551. PubMed
Product Citations
  1. Ramakrishna C, et al. 2019. Nat Commun. 10:2153. PubMed
  2. Haratani K, et al. 2019. J Clin Invest. 130:374. PubMed
  3. Wang D, et al. 2018. Immunity. 48:659. PubMed
  4. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  5. Pokrovskii M, et al. 2020. EMBO J. 39:e104159. PubMed
  6. Trott DW, et al. 2021. Geroscience. 43:1331. PubMed
  7. Sugimoto C, et al. 2022. Elife. 11:. PubMed
  8. Ayasoufi K, et al. 2020. Brain. 3629:143. PubMed
  9. Alissafi T, et al. 2020. Cell Metabolism. 32(4):591-604.e7. PubMed
  10. Miyazaki M et al. 2017. Immunity. 46(5):818-834 . PubMed
  11. Ramos CV, et al. 2020. Cell Reports. 32(3):107910. PubMed
  12. Yin Q, et al. 2021. Proc Natl Acad Sci U S A. 118: . PubMed
  13. Friedman DJ, et al. 2021. Cancer Immunol Res. 9:952. PubMed
  14. Takeshita Y, et al. 2021. Neurol Neuroimmunol Neuroinflamm. 8:. PubMed
  15. Vogel A, et al. 2022. STAR Protoc. 3:101653. PubMed
  16. Galle-Treger L, et al. 2016. Nat Commun. 7:13202. PubMed
  17. Efremova M, et al. 2018. Nat Commun. 9:32. PubMed
  18. Klepsch V, et al. 2018. Nat Commun. 9:1538. PubMed
  19. de Picciotto S, et al. 2022. Nat Commun. 13:3866. PubMed
  20. Compeer EB, et al. 2018. Nat Commun. 9:1597. PubMed
  21. Zhu S, et al. 2022. EBioMedicine. 80:104060. PubMed
  22. Damgaard RB et al. 2016. Cell. 166(5):1215-1230 . PubMed
  23. Fan MY et al. 2018. Cell reports. 25(5):1204-1213 . PubMed
  24. Coley WD et al. 2018. F1000Research. 2018 Mar 14 [revised 2018 Jan 1] 0.5125. PubMed
  25. Vogel A, et al. 2022. Cell Rep. 38:110420. PubMed
  26. Knox T, et al. 2019. Sci Rep. 9:6136. PubMed
  27. Field CS, et al. 2020. Cell Metab. 31:422. PubMed
  28. Miao Y et al. 2019. Cell. 177(5):1172-1186 . PubMed
  29. Schick V, et al. 2014. Gut. 63:1469. PubMed
RRID
AB_10895908 (BioLegend Cat. No. 102033) AB_2562611 (BioLegend Cat. No. 102043) AB_11203373 (BioLegend Cat. No. 102034)

Antigen Details

Structure
Forms high affinity IL-2R with IL-2Rβ (CD122) and IL-2Rγ (CD132), 55 kD
Distribution

Activated T cells and B cells, thymocyte subset, pre-B cells, T regulatory cells

Function
IL-2 receptor
Ligand/Receptor
IL-2
Cell Type
B cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References
  1. Taniguchi T, et al. 1993. Cell 73:5-8.
  2. Waldmann TA. 1991. J?Biol?Chem. 266:2681-4.
  3. Read S, et al. 2000. J Exp?Med. 192:295-302.
  4. Lowenthal JW, et al. 1985. J?Immunol. 135:3988-94.
Gene ID
16184 View all products for this Gene ID
UniProt
View information about CD25 on UniProt.org

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