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Biotin anti-mouse CD25 Antibody
产品名称：
Biotin anti-mouse CD25 Antibody
产品类别：
抗体
产品编号：
102003
产品应用：
102003
[价格]
规格 价格 库存
50µg ￥ 910 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
IL-2-dependent cytolytic mouse T-cell clone B6.1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, in vitro blocking of IL-2 binding to low- and high-affinity receptors1-4, growth inhibition of IL-2-dependent T-cell lines1-4, in vivo depletion of CD25+CD4+ Treg cells5-8,10, and immunohistochemical staining of acetone-fixed frozen sections2. PC61 antibody recognizes a different epitope than 3C7 antibody (Cat. No. 101902).?For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 102040) with endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered.?

Application References

(PubMed link indicates BioLegend citation)
  1. Lowenthal JW, et al. 1985. Nature 315:669. (IP, Block)
  2. Ceredig R, et al. 1985. Nature 314:98. (IP, IHC, Block)
  3. Lowenthal JW, et al. 1985. J. Immunol. 135:3988. (Block)
  4. Moreau JL, et al. 1987. Eur. J. Immunol. 17:929. (Block)
  5. Takahashi T, et al. 2000. J. Exp. Med. 192:303. (Deplete)
  6. Onizuka S, et al. 1999. Cancer Res. 59:3128. (Deplete)
  7. Lei TC, et al. 2005. Blood 105:4865. (Deplete)
  8. Pasare C, et al. 2004. Immunity 21:733. (Deplete)
  9. León-Ponte M, et al. 2007. Blood 109:3139.
  10. Cao OW, et al. 2007. Blood doi:10.1182/blood-2007-02-073304. (Deplete)
  11. Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719.
  12. Liu F, et al. 2011. Arch Toxicol. 85:1383. PubMed
  13. Anguela XM, et al. 2013. Diabetes. 62:551. PubMed
Product Citations
  1. Guo F, et al. 2022. Front Immunol. 13:833100. PubMed
  2. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  3. Xu X, et al. 2020. J Cell Biol. 219:00:00. PubMed
  4. Fukushima A, et al. 2006. Biochem Biophys Res Commun. 339:1046. PubMed
  5. Vural A, et al. 2019. J Immunol. 202:1510. PubMed
  6. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  7. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
  8. Martínez‐López M et al. 2019. Immunity. 50(2):446-461 . PubMed
  9. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  10. Akkaya B, et al. 2017. J Immunol Methods. 441:67. PubMed
  11. Frohner IE, et al. 2020. Cell Rep. 30:3171. PubMed
  12. Izumi G, et al. 2021. Nat Commun. 12:5029. PubMed
  13. Nasarre P, et al. 2021. Cancers (Basel). 13: . PubMed
  14. Miyauchi K, et al. 2016. Nat Immunol. 17:1447-1458. PubMed
RRID
AB_312852 (BioLegend Cat. No. 102003) AB_312853 (BioLegend Cat. No. 102004)

Antigen Details

Structure
Forms high affinity IL-2R with IL-2Rβ (CD122) and IL-2Rγ (CD132), 55 kD
Distribution

Activated T cells and B cells, thymocyte subset, pre-B cells, T regulatory cells

Function
IL-2 receptor
Ligand/Receptor
IL-2
Cell Type
B cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References
  1. Taniguchi T, et al. 1993. Cell 73:5-8.
  2. Waldmann TA. 1991. J?Biol?Chem. 266:2681-4.
  3. Read S, et al. 2000. J Exp?Med. 192:295-302.
  4. Lowenthal JW, et al. 1985. J?Immunol. 135:3988-94.
Gene ID
16184 View all products for this Gene ID
UniProt
View information about CD25 on UniProt.org

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