热线电话: 021-34661275
产品分类/PRODUCT CLASSIFICATION

上海细胞库

人源细胞系| 稳转细胞系| 基因敲除株| 基因点突变细胞株| 基因过表达细胞株| 重组细胞系| 猪的细胞系| 马细胞系| 兔的细胞系| 犬的细胞系| 山羊的细胞系| 鱼的细胞系| 猴的细胞系| 仓鼠的细胞系| 狗的细胞系| 牛的细胞| 大鼠细胞系| 小鼠细胞系| 其他细胞系|

原代细胞

原代细胞| 永生化细胞|

细胞培养

血清| 无血清冻存| 细胞培养试剂盒| 普通培养基| 完全培养基| 细胞污染预防| 细胞污染清除| 细胞培养辅助试剂|

细胞服务

STR鉴定服务| ATCC代购服务|

酶联试剂盒

人ELISA试剂盒| 大鼠ELISA试剂盒| 小鼠ELISA试剂盒| 兔ELISA试剂盒| 猪ELISA试剂盒| 鱼ELISA试剂盒| 牛ELISA试剂盒| 犬ELISA试剂盒| 鸭ELISA试剂盒| 豚鼠ELISA试剂盒| 鸡ELISA试剂盒| 绵羊ELISA试剂盒| 山羊ELISA试剂盒| 仓鼠ELISA试剂盒| 植物ELISA试剂盒| 药残留类试剂盒| 其他ELISA试剂盒|

生化试剂盒

生化试剂盒| 微量法检测盒| 分光法检测盒| 胶体金检测盒|

PCR试剂盒

荧光探针PCR试剂盒| 染料荧光PCR试剂盒| 核酸扩增/纯化| 普通PCR检测试剂盒| 质粒菌种感受态|

抗体蛋白

蛋白多肽| 抗体| 免疫组化| 免疫球蛋白|

试剂耗材

标准试液| 科研试剂| 缓 冲 液| 实验耗材| 高效液相色谱对照品|

首页 > 抗体蛋白 > 抗体
APC anti-mouse CD25 Antibody
产品名称：
APC anti-mouse CD25 Antibody
产品类别：
抗体
产品编号：
102011
产品应用：
102011
[价格]
规格 价格 库存
25µg ￥ 756 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
IL-2-dependent cytolytic mouse T-cell clone B6.1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The CD25 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, in vitro blocking of IL-2 binding to low- and high-affinity receptors1-4, growth inhibition of IL-2-dependent T-cell lines1-4, in vivo depletion of CD25+CD4+ Treg cells5-8,10, and immunohistochemical staining of acetone-fixed frozen sections2. PC61 antibody recognizes a different epitope than 3C7 antibody (Cat. No. 101902).?For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 102040) with endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered.?

Application References

(PubMed link indicates BioLegend citation)
  1. Lowenthal JW, et al. 1985. Nature 315:669. (IP, Block)
  2. Ceredig R, et al. 1985. Nature 314:98. (IP, IHC, Block)
  3. Lowenthal JW, et al. 1985. J. Immunol. 135:3988. (Block)
  4. Moreau JL, et al. 1987. Eur. J. Immunol. 17:929. (Block)
  5. Takahashi T, et al. 2000. J. Exp. Med. 192:303. (Deplete)
  6. Onizuka S, et al. 1999. Cancer Res. 59:3128. (Deplete)
  7. Lei TC, et al. 2005. Blood 105:4865. (Deplete)
  8. Pasare C, et al. 2004. Immunity 21:733. (Deplete)
  9. León-Ponte M, et al. 2007. Blood 109:3139.
  10. Cao OW, et al. 2007. Blood doi:10.1182/blood-2007-02-073304. (Deplete)
  11. Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719.
  12. Liu F, et al. 2011. Arch Toxicol. 85:1383. PubMed
  13. Anguela XM, et al. 2013. Diabetes. 62:551. PubMed
Product Citations
  1. Schuster M, et al. 2017. J Immunol. 199:920. PubMed
  2. Tian J, et al. 2021. Autophagy. 17:2586. PubMed
  3. Shi L, et al. 2019. Theranostics. 9:4115. PubMed
  4. Lyons J, et al. 2018. PLoS Biol. 16:e2002417. PubMed
  5. Prajeeth C,et al. 2017. J Neuroinflammation.. 10.1186/s12974-017-0978-3. PubMed
  6. Otano I, et al. 2021. Nat Commun. 12:7296. PubMed
  7. Koh CH, et al. 2020. Cancer Immunol Res. 8:698. PubMed
  8. Kolbus D, et al. 2011. Immunobiology. 216:663. PubMed
  9. Noris M, et al. 2009. J Immunol. 183:4249. PubMed
  10. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  11. Waysbort N, et al. 2013. J Immunol. 191:5822. PubMed
  12. Gravano D, et al. 2010. PLoS One. 5:e13528. PubMed
  13. Annu K, et al. 2020. Sci Rep. 10:2359. PubMed
  14. Cheng G, et al. 2021. iScience. 24:102673. PubMed
  15. Shi L, et al. 2021. Immunity. . PubMed
  16. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  17. Kang TH, et al. 2019. Tissue Eng Regen Med. 16:539. PubMed
  18. Sugimoto C, et al. 2022. Elife. 11:. PubMed
  19. Stahlhut M, et al. 2021. Sci Rep. 11:5294. PubMed
  20. Horikoshi M, et al. 2012. PLoS One. 7:e51215. PubMed
  21. Min H, et al. 2015. PLoS One. 10: 0138201. PubMed
  22. Jassinskaja M, et al. 2021. Cell Reports. 34(12):108894. PubMed
  23. Zimmermann J, et al. 2017. Eur J Immunol. 10.1002/eji.201747100. PubMed
  24. Rengarajan S, et al. 2020. Cell Rep Med. :1. PubMed
  25. Bertino P, et al. 2019. Oncoimmunology. 8:1601482. PubMed
  26. Zhang B, et al. 2021. Nat Biomed Eng. 5:1288. PubMed
  27. Park D, et al. 2020. Cancer Res. 80:4172. PubMed
  28. Yue X, et al. 2019. Nat Commun. 10:2011. PubMed
  29. Wang SS, et al. 2021. Biomedicines. 9:. PubMed
  30. Russler-Germain EV, et al. 2021. Elife. 10:. PubMed
  31. Zhong W, et al. 2022. Nat Commun. 13:4390. PubMed
  32. Chin AL, et al. 2021. Nat Commun. 12:5138. PubMed
  33. Volberding PJ, et al. 2021. Cell Reports. 35(8):109160. PubMed
  34. Glassman CR, et al. 2021. eLife. 10:00. PubMed
  35. Dou G, et al. 2020. Sci Adv. 6:eaba2987. PubMed
  36. Jing Y, et al. 2020. Sci Adv. 6:eaax9455. PubMed
  37. Kim JH, et al. 2019. Mol Med. 25:33. PubMed
  38. Bergin SM, et al. 2021. Brain Behav Immun. 95:477. PubMed
  39. Zeng Q, et al. 2022. Front Immunol. 13:740805. PubMed
  40. Wigren M, et al. 2011. J Intern Med. 269:546. PubMed
  41. Vaena S, et al. 2021. Cell Reports. 35(5):109076. PubMed
  42. Pierini A, et al. 2017. JCI Insight. 2. PubMed
  43. Kemp V, et al. 2018. Cancer Gene Ther. 26:268. PubMed
  44. Shen M, et al. 2022. Nat Cancer. 3:60. PubMed
  45. Webb ER, et al. 2022. iScience. 25:104995. PubMed
  46. Glassman CR, et al. 2021. Cell. 184(4):983-999.e24. PubMed
  47. Oomizu S, et al. 2012. Clin Immunol. 143:51. PubMed
  48. Liu X, et al. 2021. Int J Nanomedicine. 16:5675. PubMed
  49. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
  50. Wei Z, et al. 2021. Nat Commun. 0.805555556. PubMed
  51. Kolev M, et al. 2020. Immunity. 52(3):513-527. PubMed
  52. Aftabizadeh M, et al. 2021. JCI Insight. 6:. PubMed
  53. Xu Q, et al. 2021. Theranostics. 11:1937. PubMed
  54. Kang CK, et al. 2021. J Infect Dis. 224:39. PubMed
  55. Tu J, et al. 2022. Mol Ther Nucleic Acids. 27:733. PubMed
  56. Martomo SA, et al. 2021. Mol Cancer Ther. 1.074305556. PubMed
  57. Long C, et al. 2016. Toxicol Sci. 152: 85 - 98. PubMed
  58. Baomei Wang et al. 2019. Cell reports. 26(6):1614-1626 . PubMed
  59. Nenasheva T, et al. 2017. PLoS One. 12(6):e0178983. PubMed
  60. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  61. Ahn SH, et al. 2022. Front Endocrinol (Lausanne). 13:846226. PubMed
  62. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
  63. Pardo E, et al. 2017. PLoS One. 12(6):e0177472. PubMed
  64. Wang T et al. 2018. Immunity. 49(3):504-514 . PubMed
  65. Barbet G, et al. 2018. Immunity. 48:584. PubMed
  66. Chen X, et al. 2019. Med Sci Monit. 25:5389. PubMed
  67. Yue X, et al. 2021. EMBO Rep. 22:e52716. PubMed
  68. O'Donoghue GP, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  69. Tsai HI, et al. 2021. EMBO Mol Med. 13:e12834. PubMed
  70. Hoover AR, et al. 2022. Clin Transl Med. 12:e937. PubMed
  71. Tanaka S, et al. 2016. Appl Envir Microbiol. 82: 2693 - 2699. PubMed
  72. Chen C, et al. 2020. Front Cell Neurosci. 14:8. PubMed
  73. Niven J, et al. 2019. Cell Rep. 28:21. PubMed
  74. Schuster M, et al. 2019. Front Immunol. 10:1583. PubMed
  75. Alenazy MF, et al. 2022. PLoS One. 17:e0271689. PubMed
  76. Shaikh H, et al. 2021. Front Immunol. 12:689896. PubMed
  77. Zheng Y, et al. 2022. J Immunol. 208:501. PubMed
  78. Tassi I, et al. 2014. J Immunol. 193:4303. PubMed
  79. Wong E, et al. 2015. J Immunol. 194:4130. PubMed
  80. Yang X, et al. 2021. Bioact Mater. 3150:6. PubMed
  81. Chao JL, et al. 2021. Cell Rep Med. 2:100399. PubMed
  82. Sun Y, et al. 2022. iScience. 25:104846. PubMed
  83. Schulze J, et al. 2021. Stroke. 52:2939. PubMed
  84. Katsuyama T, et al. 2021. Cell Reports. 36(1):109339. PubMed
  85. Kashiwakura Y, et al. 2020. Clinical & Experimental Immunology. 202(1):119-135. PubMed
  86. Deptuch T, et al. 2022. Sci Rep. 12:13480. PubMed
  87. Du Z, et al. 2020. J Allergy Clin Immunol. . PubMed
  88. Ye Y, et al. 2020. Genome Med. 0.557638889. PubMed
  89. Angelou CC, et al. 2020. Frontiers in Immunology. 2.586805556. PubMed
  90. Johnson JL et al. 2018. Immunity. 48(2):243-257 . PubMed
  91. Li CY, et al. 2022. Int J Mol Sci. 23:. PubMed
  92. Erens C, et al. 2022. Biomedicines. 10:. PubMed
  93. Park JS, et al. 2022. J Transl Med. 20:85. PubMed
  94. Rana MN, et al. 2021. Front Pharmacol. 12:643215. PubMed
RRID
AB_312860 (BioLegend Cat. No. 102011) AB_312861 (BioLegend Cat. No. 102012)

Antigen Details

Structure
Forms high affinity IL-2R with IL-2Rβ (CD122) and IL-2Rγ (CD132), 55 kD
Distribution

Activated T cells and B cells, thymocyte subset, pre-B cells, T regulatory cells

Function
IL-2 receptor
Ligand/Receptor
IL-2
Cell Type
B cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References
  1. Taniguchi T, et al. 1993. Cell 73:5-8.
  2. Waldmann TA. 1991. J?Biol?Chem. 266:2681-4.
  3. Read S, et al. 2000. J Exp?Med. 192:295-302.
  4. Lowenthal JW, et al. 1985. J?Immunol. 135:3988-94.
Gene ID
16184 View all products for this Gene ID
UniProt
View information about CD25 on UniProt.org

联系我们

TEL：021-34661275 　点击拨打热线