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Alexa Fluor® 700 anti-mouse CD25 Antibody
产品名称：
Alexa Fluor® 700 anti-mouse CD25 Antibody
产品类别：
抗体
产品编号：
102024
产品应用：
102024
[价格]
规格 价格 库存
100µg ￥ 3206 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
IL-2-dependent cytolytic mouse T-cell clone B6.1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis.?The suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 700 has a maximum emission of 719 nm when it is excited at 633nm / 635nm. Prior to using Alexa Fluor? 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, in vitro blocking of IL-2 binding to low- and high-affinity receptors1-4, growth inhibition of IL-2-dependent T-cell lines1-4, in vivo depletion of CD25+CD4+ Treg cells5-8,10, and immunohistochemical staining of acetone-fixed frozen sections2. PC61 antibody recognizes a different epitope than 3C7 antibody (Cat. No. 101902).?For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 102040) with endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered.?

Application References

(PubMed link indicates BioLegend citation)
  1. Lowenthal JW, et al. 1985. Nature 315:669. (IP, Block)
  2. Ceredig R, et al. 1985. Nature 314:98. (IP, IHC, Block)
  3. Lowenthal JW, et al. 1985. J. Immunol. 135:3988. (Block)
  4. Moreau JL, et al. 1987. Eur. J. Immunol. 17:929. (Block)
  5. Takahashi T, et al. 2000. J. Exp. Med. 192:303. (Deplete)
  6. Onizuka S, et al. 1999. Cancer Res. 59:3128. (Deplete)
  7. Lei TC, et al. 2005. Blood 105:4865. (Deplete)
  8. Pasare C, et al. 2004. Immunity 21:733. (Deplete)
  9. León-Ponte M, et al. 2007. Blood 109:3139.
  10. Cao OW, et al. 2007. Blood doi:10.1182/blood-2007-02-073304. (Deplete)
  11. Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719.
  12. Liu F, et al. 2011. Arch Toxicol. 85:1383. PubMed
  13. Anguela XM, et al. 2013. Diabetes. 62:551. PubMed
Product Citations
  1. Lu Y, et al. 2011. PLoS One. 6:e19889. PubMed
  2. Petrova T, et al. 2020. Sci Rep. 10:3479. PubMed
  3. Marchelletta RR, et al. 2021. J Clin Invest. 131:. PubMed
  4. Lancaster JN, et al. 2019. Nat Commun. 10:2220. PubMed
  5. Sharma NS, et al. 2020. J Clin Invest. 130:451. PubMed
  6. Tordesillas L, et al. 2018. Nat Commun. 9:5238. PubMed
  7. Shi L, et al. 2021. Immunity. . PubMed
  8. Gubser C, et al. 2016. Sci Rep. 6: 25758. PubMed
  9. Stokes J, et al. 2020. Oncoimmunology. 9:1758011. PubMed
  10. Miska J et al. 2019. Cell reports. 27(1):226-237 . PubMed
  11. Momin N, et al. 2022. Nat Commun. 13:109. PubMed
  12. Ruscher R, et al. 2018. Bio Protoc. 8:e2757. PubMed
  13. Field CS, et al. 2020. Cell Metab. 31:422. PubMed
  14. Kitagawa K, et al. 2014. Mol Cell Biol. 34:2732. PubMed
  15. Oh J, et al. 2021. Clin Cancer Res. 27:4781. PubMed
RRID
AB_493709 (BioLegend Cat. No. 102024)

Antigen Details

Structure
Forms high affinity IL-2R with IL-2Rβ (CD122) and IL-2Rγ (CD132), 55 kD
Distribution

Activated T cells and B cells, thymocyte subset, pre-B cells, T regulatory cells

Function
IL-2 receptor
Ligand/Receptor
IL-2
Cell Type
B cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References
  1. Taniguchi T, et al. 1993. Cell 73:5-8.
  2. Waldmann TA. 1991. J?Biol?Chem. 266:2681-4.
  3. Read S, et al. 2000. J Exp?Med. 192:295-302.
  4. Lowenthal JW, et al. 1985. J?Immunol. 135:3988-94.
Gene ID
16184 View all products for this Gene ID
UniProt
View information about CD25 on UniProt.org

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