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首页 > 抗体蛋白 > 抗体
Purified anti-human/mouse/rat PCNA Antibody
产品名称：
Purified anti-human/mouse/rat PCNA Antibody
产品类别：
抗体
产品编号：
307901
产品应用：
307901
[价格]
规格 价格 库存
25µg ￥ 826 1

产品详情

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant rat PCNA
Formulation
Phosphate-buffered solution, pH7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICFC - Quality tested
ICC, IHC-P?- Verified
IP, WB, IHC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent intracellular staining with flow cytometric analysis. Please follow the?Cell Fixation and Permeabilization Protocol Using 70% Ethanol.?For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 ?g per million cells in 100 ?L?volume.?It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining2,5,6 of acetone-fixed frozen sections and formalin-fixed paraffin-embedded tissue sections, immunoprecipitation, intracellular flow cytometry3, immunofluorescence microscopy9, and Western blotting10.

Application References

(PubMed link indicates BioLegend citation)
  1. Ogata K, et al. 1985. J. Immunol. 135:2623.
  2. Garcia R, et al. 1989. Am. J. Pathol. 134:733.
  3. Landberg G, et al. 1990. Exp. Cell. Res. 187:111.
  4. Waseem N, et al. 1990. J. Cell Sci. 96:121.
  5. Yu C, et al. 1991. Histopathology 19:29.
  6. Wilkins B, et al. 1992. J. Pathol. 166:45.
  7. Yang W, et al. 1996. Human Pathol. 27:70.
  8. Galkowska H, et al. 1996. Vet. Immunol. Immunopathol. 53:329.
  9. Chou HYE, et al. 2006. J. Biol. Chem. 10:1074.
  10. Fulvio MD, et al. 2006. Oncogene 25:3932.
  11. Eswarakumar VP and Schlessinger J. 2007. Proc. Natl. Acad. Sci. USA 104:3937.
  12. Henkels KM, et al. 2009. Biochem Biophys Res Commun. 389:224. PubMed
  13. Brobeli A, et al. 2010. Blood Cells Mol Dis. 45:159. PubMed
  14. Wallace HA, et al. 2014. Development. 141:1332. PubMed
  15. Mizokami A, et al. 2014. Bone. 69:68. PubMed
Product Citations
  1. Ho EK, et al. 2020. Current Biology. 30(14):2829-2835.e5. PubMed
  2. Mizokami A, et al. 2014. Bone. 69:68. PubMed
  3. Eymann J, et al. 2020. Frontiers in Cell and Developmental Biology. 0.615277778. PubMed
  4. Salomies L, et al. 2021. Sci Adv. 7:eabj7912. PubMed
  5. Moreno–Marín N, et al. 2018. iScience. 4:44. PubMed
  6. Brobeil A, et al. 2010. Blood Cells Mol Dis. 45:159. PubMed
  7. Brummer G, et al. 2020. Oncogene. 39:2275. PubMed
  8. Wallace H, et al. 2014. Development. 141:1332. PubMed
  9. Salomies L et al. 2019. Elife. 8 pii: e47702. PubMed
  10. Schlessinger V 2007. Proc Natl Acad Sci U S A. 104:3937. PubMed
  11. Eymann J et al. 2019. The Journal of comparative neurology. 527(14):2356-2370 . PubMed
  12. Moreno-Marín N, et al. 2017. Sci Rep. 10.1038/s41598-017-10984-w. PubMed
  13. Gal O, et al. 2022. J Nanotheranostics. 3:177. PubMed
  14. Hu Q, et al. 2019. Biol Open. 8. PubMed
  15. Pepe‐Mooney BJ et al. 2019. Cell Stem Cell. 25(1):23-38 . PubMed
  16. Padavannil A, et al. 2019. Elife. 8. PubMed
  17. Fang WB, et al. 2021. Sci Rep. 11:8708. PubMed
  18. Tsai HH, et al. 2020. Sci Rep. 0.640277778. PubMed
  19. Henkels K, et al. 2009. Biochem Biophys Res Commun. 389:224. PubMed
  20. Lan T, et al. 2015. PLoS One. 10: 0129743. PubMed
  21. Perini S, et al. 2016. Rev Port Pneumol (2006). 22:209-213. PubMed
  22. Wang JT et al. 2017. eLife. 6 pii: e29061. PubMed
  23. Wang Q, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
RRID
AB_314691 (BioLegend Cat. No. 307901) AB_314692 (BioLegend Cat. No. 307902)

Antigen Details

Structure
DNA-polymerase sliding clamp, trimeric ring; 36 kD
Distribution

Nuclear, all proliferating cells

Function
RAD6-dependent DNA repair pathway; increases DNA polymerase δ processibility during elongation of the leading strand
Interaction
PCNA, DNA polymerase δ, Rad6, Rad18, UBC9, MMS2, UBC13, RAD5
Ligand/Receptor
Ubiquitination, Sumoylation
Cell Type
Neural Stem Cells
Biology Area
Cell Biology, Cell Cycle/DNA Replication, DNA Repair/Replication, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Nuclear Markers
Antigen References

1. Travali S, et al. 1989. J. Biol. Chem. 264:7466.
2. Waseem N, et al. 1990. J. Cell Sci. 96:121.
3. Hall P, et al. 1990. J. Pathol. 162:285.
4. Landberg G, et al. 1991. Cancer Res. 51:4570.
5. Woods A, et al. 1991. Histopathol. 19:21.
6. Hoege C, et al. 2002. Nature 419:135.
7. Yue H, et al. 2003. World J. Gastroenterol. 9:377.
8. Shan B, et al. 2003. J. Biol. Chem. 278:44009.

Gene ID
5111 View all products for this Gene ID
UniProt
View information about PCNA on UniProt.org

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