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首页 > 抗体蛋白 > 抗体
Biotin anti-human/mouse/rat PCNA Antibody
产品名称：
Biotin anti-human/mouse/rat PCNA Antibody
产品类别：
抗体
产品编号：
307904
产品应用：
307904
[价格]
规格 价格 库存
100µg ￥ 3164 1

产品详情

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant rat PCNA
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent intracellular staining with flow cytometric analysis. Please follow the?Cell Fixation and Permeabilization Protocol Using 70% Ethanol.?For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 ?g per million cells in 100 ?L?volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining2,5,6 of acetone-fixed frozen sections and formalin-fixed paraffin-embedded tissue sections, immunoprecipitation, intracellular flow cytometry3, immunofluorescence microscopy9, and Western blotting10.

Application References

(PubMed link indicates BioLegend citation)
  1. Ogata K, et al. 1985. J. Immunol. 135:2623.
  2. Garcia R, et al. 1989. Am. J. Pathol. 134:733.
  3. Landberg G, et al. 1990. Exp. Cell. Res. 187:111.
  4. Waseem N, et al. 1990. J. Cell Sci. 96:121.
  5. Yu C, et al. 1991. Histopathology 19:29.
  6. Wilkins B, et al. 1992. J. Pathol. 166:45.
  7. Yang W, et al. 1996. Human Pathol. 27:70.
  8. Galkowska H, et al. 1996. Vet. Immunol. Immunopathol. 53:329.
  9. Chou HYE, et al. 2006. J. Biol. Chem. 10:1074.
  10. Fulvio MD, et al. 2006. Oncogene 25:3932.
  11. Eswarakumar VP and Schlessinger J. 2007. Proc. Natl. Acad. Sci. USA 104:3937.
  12. Henkels KM, et al. 2009. Biochem Biophys Res Commun. 389:224. PubMed
  13. Brobeli A, et al. 2010. Blood Cells Mol Dis. 45:159. PubMed
  14. Wallace HA, et al. 2014. Development. 141:1332. PubMed
  15. Mizokami A, et al. 2014. Bone. 69:68. PubMed
Product Citations
  1. Spector I, et al. 2012. PLoS One. 7:e41833. PubMed
  2. Zacarias-Fluck MF, et al. 2021. Life Sci Alliance. :4. PubMed
  3. Begolly S, et al. 2018. Glia. 66:846. PubMed
  4. Wang L, et al. 2022. Nat Commun. 13:4709. PubMed
  5. Satchi-Fainaro R, et al. 2012. PLoS One. 7:e44395. PubMed
  6. Dopeso H, et al. 2009. Cancer Res. 69:7430. PubMed
  7. Yan H, et al. 2019. Sci Rep. 9:10447. PubMed
  8. Kim J, et al. 2012. Immunol Lett. 147:18. PubMed
RRID
AB_314694 (BioLegend Cat. No. 307904)

Antigen Details

Structure
DNA-polymerase sliding clamp, trimeric ring; 36 kD
Distribution

Nuclear, all proliferating cells

Function
RAD6-dependent DNA repair pathway; increases DNA polymerase δ processibility during elongation of the leading strand
Interaction
PCNA, DNA polymerase δ, Rad6, Rad18, UBC9, MMS2, UBC13, RAD5
Ligand/Receptor
Ubiquitination, Sumoylation
Cell Type
Neural Stem Cells
Biology Area
Cell Biology, Cell Cycle/DNA Replication, DNA Repair/Replication, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Nuclear Markers
Antigen References

1. Travali S, et al. 1989. J. Biol. Chem. 264:7466.
2. Waseem N, et al. 1990. J. Cell Sci. 96:121.
3. Hall P, et al. 1990. J. Pathol. 162:285.
4. Landberg G, et al. 1991. Cancer Res. 51:4570.
5. Woods A, et al. 1991. Histopathol. 19:21.
6. Hoege C, et al. 2002. Nature 419:135.
7. Yue H, et al. 2003. World J. Gastroenterol. 9:377.
8. Shan B, et al. 2003. J. Biol. Chem. 278:44009.

Gene ID
5111 View all products for this Gene ID
UniProt
View information about PCNA on UniProt.org

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