Alexa Fluor® 594 anti-human/mouse/rat PCNA Ant-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 3710	1

Product Details

Verified Reactivity

Human, Mouse, Rat

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Recombinant rat PCNA

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 594 under optimal conditions.

Concentration

0.5 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICC?- Quality tested
IHC-P - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL?is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 1.0 - 5.0 μg/mL?is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses

Excitation Laser

Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining^2,5,6 of acetone-fixed frozen sections and formalin-fixed paraffin-embedded tissue sections, immunoprecipitation, intracellular flow cytometry³, immunofluorescence microscopy⁹, and Western blotting¹⁰.

Application References (PubMed link indicates BioLegend citation)

Ogata K, et al. 1985. J. Immunol. 135:2623.
Garcia R, et al. 1989. Am. J. Pathol. 134:733.
Landberg G, et al. 1990. Exp. Cell. Res. 187:111.
Waseem N, et al. 1990. J. Cell Sci. 96:121.
Yu C, et al. 1991. Histopathology 19:29.
Wilkins B, et al. 1992. J. Pathol. 166:45.
Yang W, et al. 1996. Human Pathol. 27:70.
Galkowska H, et al. 1996. Vet. Immunol. Immunopathol. 53:329.
Chou HYE, et al. 2006. J. Biol. Chem. 10:1074.
Fulvio MD, et al. 2006. Oncogene 25:3932.
Eswarakumar VP and Schlessinger J. 2007. Proc. Natl. Acad. Sci. USA 104:3937.
Henkels KM, et al. 2009. Biochem Biophys Res Commun. 389:224. PubMed
Brobeli A, et al. 2010. Blood Cells Mol Dis. 45:159. PubMed
Wallace HA, et al. 2014. Development. 141:1332. PubMed
Mizokami A, et al. 2014. Bone. 69:68. PubMed

RRID

AB_2563317 (BioLegend Cat. No. 307914)

Antigen Details

Structure: DNA-polymerase sliding clamp, trimeric ring; 36 kD
Distribution: Nuclear, all proliferating cells
Function: RAD6-dependent DNA repair pathway; increases DNA polymerase δ processibility during elongation of the leading strand
Interaction: PCNA, DNA polymerase δ, Rad6, Rad18, UBC9, MMS2, UBC13, RAD5
Ligand/Receptor: Ubiquitination, Sumoylation
Cell Type: Neural Stem Cells
Biology Area: Cell Biology, Cell Cycle/DNA Replication, DNA Repair/Replication, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: Nuclear Markers
Antigen References: 1. Travali S, et al. 1989. J. Biol. Chem. 264:7466.
2. Waseem N, et al. 1990. J. Cell Sci. 96:121.
3. Hall P, et al. 1990. J. Pathol. 162:285.
4. Landberg G, et al. 1991. Cancer Res. 51:4570.
5. Woods A, et al. 1991. Histopathol. 19:21.
6. Hoege C, et al. 2002. Nature 419:135.
7. Yue H, et al. 2003. World J. Gastroenterol. 9:377.
8. Shan B, et al. 2003. J. Biol. Chem. 278:44009.
Gene ID: 5111 View all products for this Gene ID
UniProt: View information about PCNA on UniProt.org

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