Alexa Fluor® 488 anti-human/mouse/rat PCNA Ant-上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 1736	1

Product Details

Verified Reactivity

Human, Mouse, Rat

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Recombinant rat PCNA

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. Please follow the Cell Fixation and Permeabilization Protocol Using 70% Ethanol. For flow cytometric staining, the suggested use of this reagent is 5 ?L?per million cells in 100 ?L?staining volume or 5 ?L?per 100 ?L?of whole blood.

* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses

Excitation Laser

Blue Laser (488 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining^2,5,6 of acetone-fixed frozen sections and formalin-fixed paraffin-embedded tissue sections, immunoprecipitation, intracellular flow cytometry³, immunofluorescence microscopy⁹, and Western blotting¹⁰.

Application References (PubMed link indicates BioLegend citation)

Ogata K, et al. 1985. J. Immunol. 135:2623.
Garcia R, et al. 1989. Am. J. Pathol. 134:733.
Landberg G, et al. 1990. Exp. Cell. Res. 187:111.
Waseem N, et al. 1990. J. Cell Sci. 96:121.
Yu C, et al. 1991. Histopathology 19:29.
Wilkins B, et al. 1992. J. Pathol. 166:45.
Yang W, et al. 1996. Human Pathol. 27:70.
Galkowska H, et al. 1996. Vet. Immunol. Immunopathol. 53:329.
Chou HYE, et al. 2006. J. Biol. Chem. 10:1074.
Fulvio MD, et al. 2006. Oncogene 25:3932.
Eswarakumar VP and Schlessinger J. 2007. Proc. Natl. Acad. Sci. USA 104:3937.
Henkels KM, et al. 2009. Biochem Biophys Res Commun. 389:224. PubMed
Brobeli A, et al. 2010. Blood Cells Mol Dis. 45:159. PubMed
Wallace HA, et al. 2014. Development. 141:1332. PubMed
Mizokami A, et al. 2014. Bone. 69:68. PubMed

Product Citations

Horton N, et al. 2013. PLoS One. 8:e59552. PubMed

RRID

AB_2160546 (BioLegend Cat. No. 307909)

Antigen Details

Structure: DNA-polymerase sliding clamp, trimeric ring; 36 kD
Distribution: Nuclear, all proliferating cells
Function: RAD6-dependent DNA repair pathway; increases DNA polymerase δ processibility during elongation of the leading strand
Interaction: PCNA, DNA polymerase δ, Rad6, Rad18, UBC9, MMS2, UBC13, RAD5
Ligand/Receptor: Ubiquitination, Sumoylation
Cell Type: Neural Stem Cells
Biology Area: Cell Biology, Cell Cycle/DNA Replication, DNA Repair/Replication, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: Nuclear Markers
Antigen References: 1. Travali S, et al. 1989. J. Biol. Chem. 264:7466.
2. Waseem N, et al. 1990. J. Cell Sci. 96:121.
3. Hall P, et al. 1990. J. Pathol. 162:285.
4. Landberg G, et al. 1991. Cancer Res. 51:4570.
5. Woods A, et al. 1991. Histopathol. 19:21.
6. Hoege C, et al. 2002. Nature 419:135.
7. Yue H, et al. 2003. World J. Gastroenterol. 9:377.
8. Shan B, et al. 2003. J. Biol. Chem. 278:44009.
Gene ID: 5111 View all products for this Gene ID
UniProt: View information about PCNA on UniProt.org

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