Alexa Fluor® 647 anti-human CD41/CD61 Antibody-上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 2310	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Human platelets

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Excitation Laser

Red Laser (633 nm)

Application Notes

Additional reported applications (for the relevant formats of this clone) include: immunoprecipitation^1,2, Western blotting²and in vitro blocking of platelet aggregation².

The PAC-1 antibody reacts with activation-induced conformational epitope of the integrin a_IIb?_3.PAC-1 binding to fibrinogen is pH and Ca²⁺ dependent, and will not bind to platelets that have been fixed.? We recommend staining in buffers free of Ethylenediaminetetraacetic acid (EDTA) at a pH of 6.5 or higher. ?Sodium citrate may be used as an anticoagulant for blood collection³.

Application References (PubMed link indicates BioLegend citation)

Shattil SJ, et al. 1987. Blood. 70:307. (IP)
Shattil SJ, et al. 1985. J. Biol. Chem. 260:11107. (Block, IP, WB)
Shattil SJ, et al. 1986. Blood. 68: 1224-31.

Product Citations

Chauhan A, et al. 2020. Nat Commun. 11:1939. PubMed
Nicolai L, et al. 2020. Circulation. 142:1176. PubMed
Gillespie S, et al. 2018. Br J Pharmacol. 175:3333. PubMed
Bahmani B, et al. 2021. Nat Commun. 12:1999. PubMed
Chen Z, et al. 2019. Nat Commun. 10:2051. PubMed

RRID

AB_2566475 (BioLegend Cat. No. 362805) AB_2566476 (BioLegend Cat. No. 362806)

Antigen Details

Structure: α/β transmembrane heterodimer of the ubiquitous integrin superfamily
Distribution: Resting platelets; activated platelets express a conformationally modified complex that can bind fibrin
Function: Fibrinogen binding, platelet aggregation
Ligand/Receptor: Fibrinogen
Cell Type: Platelets
Biology Area: Cell Adhesion, Cell Biology, Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Hynes RO. 1992. Cell 69:11.
2. Shattil SJ, et al. 1985. J. Biol. Chem. 260:11107.
3. Ginsberg, et al. 1990. Blood 76:2017.
4. O’Toole TE, et al. 1991. Science 254:845.
5. Peterson JA, et al. 1998. Blood?92: 2053.
Gene ID: 3674 View all products for this Gene ID 3690 View all products for this Gene ID
UniProt: View information about CD41/CD61 on UniProt.org

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