Ultra-LEAF™ Purified anti-mouse CD172a (SIRP-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 1540	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Mouse brain membrane protein

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/?g of the protein (< 0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
IHC-F, IP, Block - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: blocking SIRPa interaction with CD47⁴, in vivo blocking of dendritic cell migration³, enhancing of macrophage phagocytosis^2,4, immunohistochemical staining of cerebellum frozen sections¹, immunoprecipitation^2,4, and spatial biology (IBEX)^5,6.

Application References (PubMed link indicates BioLegend citation)

Comu S, et al. 1997. J. Neurosci. 17:8702. (IHC)
Gresham HD, et al. 2000. J. Exp. Med. 191:515. (IP)
Fukunaga A, et al. 2004. J. Immunol. 172:4091. (Block)
Oldenborg PA, et al. 2000. Science 288:2051. (Block, IP)
Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed

RRID

AB_2832516 (BioLegend Cat. No. 144035) AB_2832517 (BioLegend Cat. No. 144036) AB_2832518 (BioLegend Cat. No. 144037) AB_2832519 (BioLegend Cat. No. 144038) AB_2832520 (BioLegend Cat. No. 144039) AB_2832521 (BioLegend Cat. No. 144040)

Antigen Details

Structure: Type I transmembrane protein
Distribution: Monocytes, macrophages, myeloid cells, neuronal tissue
Function: Negative regulation of several biological processes
Interaction: PTPN6, PTPN11
Ligand/Receptor: CD47, SP-A, SP-D
Cell Type: Dendritic cells, Macrophages, Monocytes, Neutrophils
Biology Area: Cell Adhesion, Cell Biology, Immunology, Signal Transduction
Molecular Family: Adhesion Molecules, CD Molecules, Protein Kinases/Phosphatase
Antigen References: 1. Zhao XW, et al. 2011. P. Natl. Acad. Sci. USA 108:18342.
2. Verjan-Garcia N, et al. 2011. J. Immunol. 187:2268.
3. Sato-Hashimoto M, et al. 2011. J. Immunol. 187:291.
4. Raymond M, et al. 2010. Eur. J. Immunol. 40:3510.
Gene ID: 19261 View all products for this Gene ID
UniProt: View information about CD172alpha; on UniProt.org

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