PE/Cyanine7 anti-mouse CD172a (SIRPα) Antibo-上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 2058	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Mouse brain membrane protein

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Additional reported applications (for the relevant formats) include: blocking SIRPa interaction with CD47⁴, in vivo blocking of dendritic cell migration³, enhancing of macrophage phagocytosis^2,4, immunohistochemical staining of cerebellum frozen sections¹, immunoprecipitation^2,4, and spatial biology (IBEX)^5,6.

Additional Product Notes

BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.

Application References (PubMed link indicates BioLegend citation)

Comu S, et al. 1997. J. Neurosci. 17:8702. (IHC)
Gresham HD, et al. 2000. J. Exp. Med. 191:515. (IP)
Fukunaga A, et al. 2004. J. Immunol. 172:4091. (Block)
Oldenborg PA, et al. 2000. Science 288:2051. (Block, IP)
Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed

Product Citations

Lancaster JN, et al. 2019. Nat Commun. 10:2220. PubMed
Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
Bosteels C, et al. 2020. Immunity. 52(6):1039-1056.e9.. PubMed

RRID

AB_2563545 (BioLegend Cat. No. 144007) AB_2563546 (BioLegend Cat. No. 144008)

Antigen Details

Structure: Type I transmembrane protein
Distribution: Monocytes, macrophages, myeloid cells, neuronal tissue
Function: Negative regulation of several biological processes
Interaction: PTPN6, PTPN11
Ligand/Receptor: CD47, SP-A, SP-D
Cell Type: Dendritic cells, Macrophages, Monocytes, Neutrophils
Biology Area: Cell Adhesion, Cell Biology, Immunology, Signal Transduction
Molecular Family: Adhesion Molecules, CD Molecules, Protein Kinases/Phosphatase
Antigen References: 1. Zhao XW, et al. 2011. P. Natl. Acad. Sci. USA 108:18342.
2. Verjan-Garcia N, et al. 2011. J. Immunol. 187:2268.
3. Sato-Hashimoto M, et al. 2011. J. Immunol. 187:291.
4. Raymond M, et al. 2010. Eur. J. Immunol. 40:3510.
Gene ID: 19261 View all products for this Gene ID
UniProt: View information about CD172alpha; on UniProt.org

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