APC/Fire™ 750 anti-mouse CD172a (SIRPα-上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 2058	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Mouse brain membrane protein

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with APC/Fire? 750 under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* APC/Fire? 750 has a maximum excitation of 650 nm and a maximum emission of 787 nm.

Excitation Laser

Red Laser (633 nm)

Application Notes

Additional reported applications (for the relevant formats) include: blocking SIRPa interaction with CD47⁴, in vivo blocking of dendritic cell migration³, enhancing of macrophage phagocytosis^2,4, immunohistochemical staining of cerebellum frozen sections¹, immunoprecipitation^2,4, and spatial biology (IBEX)^5,6.

Application References (PubMed link indicates BioLegend citation)

Comu S, et al. 1997. J. Neurosci. 17:8702. (IHC)
Gresham HD, et al. 2000. J. Exp. Med. 191:515. (IP)
Fukunaga A, et al. 2004. J. Immunol. 172:4091. (Block)
Oldenborg PA, et al. 2000. Science 288:2051. (Block, IP)
Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed

Product Citations

Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed
Giampazolias E, et al. 2021. Cell. . PubMed

RRID

AB_2721316 (BioLegend Cat. No. 144029) AB_2721317 (BioLegend Cat. No. 144030)

Antigen Details

Structure: Type I transmembrane protein
Distribution: Monocytes, macrophages, myeloid cells, neuronal tissue
Function: Negative regulation of several biological processes
Interaction: PTPN6, PTPN11
Ligand/Receptor: CD47, SP-A, SP-D
Cell Type: Dendritic cells, Macrophages, Monocytes, Neutrophils
Biology Area: Cell Adhesion, Cell Biology, Immunology, Signal Transduction
Molecular Family: Adhesion Molecules, CD Molecules, Protein Kinases/Phosphatase
Antigen References: 1. Zhao XW, et al. 2011. P. Natl. Acad. Sci. USA 108:18342.
2. Verjan-Garcia N, et al. 2011. J. Immunol. 187:2268.
3. Sato-Hashimoto M, et al. 2011. J. Immunol. 187:291.
4. Raymond M, et al. 2010. Eur. J. Immunol. 40:3510.
Gene ID: 19261 View all products for this Gene ID
UniProt: View information about CD172alpha; on UniProt.org

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