Alexa Fluor® 594 anti-mouse CD172a (SIRP&alpha-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 4760	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Mouse brain membrane protein

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 594 under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

IHC-F - Quality tested
3D IHC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 2.5 - 10 μg/ml is suggested. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 ?g/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Application Notes

Additional reported applications (for the relevant formats) include: blocking SIRPa interaction with CD47⁴, in vivo blocking of dendritic cell migration³, enhancing of macrophage phagocytosis^2,4, immunohistochemical staining of cerebellum frozen sections¹, immunoprecipitation^2,4, and spatial biology (IBEX)^5,6.

Application References (PubMed link indicates BioLegend citation)

Comu S, et al. 1997. J. Neurosci. 17:8702. (IHC)
Gresham HD, et al. 2000. J. Exp. Med. 191:515. (IP)
Fukunaga A, et al. 2004. J. Immunol. 172:4091. (Block)
Oldenborg PA, et al. 2000. Science 288:2051. (Block, IP)
Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed

RRID

AB_2629588 (BioLegend Cat. No. 144020)

Antigen Details

Structure: Type I transmembrane protein
Distribution: Monocytes, macrophages, myeloid cells, neuronal tissue
Function: Negative regulation of several biological processes
Interaction: PTPN6, PTPN11
Ligand/Receptor: CD47, SP-A, SP-D
Cell Type: Dendritic cells, Macrophages, Monocytes, Neutrophils
Biology Area: Cell Adhesion, Cell Biology, Immunology, Signal Transduction
Molecular Family: Adhesion Molecules, CD Molecules, Protein Kinases/Phosphatase
Antigen References: 1. Zhao XW, et al. 2011. P. Natl. Acad. Sci. USA 108:18342.
2. Verjan-Garcia N, et al. 2011. J. Immunol. 187:2268.
3. Sato-Hashimoto M, et al. 2011. J. Immunol. 187:291.
4. Raymond M, et al. 2010. Eur. J. Immunol. 40:3510.
Gene ID: 19261 View all products for this Gene ID
UniProt: View information about CD172alpha; on UniProt.org

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