Purified anti-Ubiquitin Antibody
- 产品名称：
- Purified anti-Ubiquitin Antibody
- 产品类别：
- 抗体
- 产品编号：
- 838703
- 产品应用：
- 838703
- Verified Reactivity
- Human, Mouse, Rat, Drosophila
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Clone P4G7 was raised against denatured bovine ubiquitin and recognizes ubiquitin, polyubiquitin, and ubiquitin-conjugated proteins.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IHC-P - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10 ?g per ml.?For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 0.5 - 5.0 ?g/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
P4G7 is equivalent to MAb P4D1. The ubiquitin protein is extremely well conserved and thus the antibody has extensive species cross-reactivity from yeast to human. This antibody was specifically developed to detect ubiquitin and ubiquitin-substrate conjugates by immunoblotting.
- Application References
(PubMed link indicates BioLegend citation) -
- Duttler S, et al. 2013. Mol. Cell. 3:379. (WB) PubMed
- Wu Y, et al. 2007. Mol. Cell. Biol. 18:6407. (WB) PubMed
- Product Citations
-
- Weskamp G, et al. 2020. J Biol Chem. 295:4350. PubMed
- Lora J, et al. 2021. J Biol Chem. :100733. PubMed
- Kumar P, et al. 2021. Journal of Cellular Biochemistry. :. PubMed
- Qui?ones JL, et al. 2020. DNA Repair (Amst). 102773:87. PubMed
- Weskamp G, et al. 2020. J Biol Chem. 295:4350. PubMed
- RRID
- AB_2734615 (BioLegend Cat. No. 838703) AB_2632643 (BioLegend Cat. No. 838704)
- Structure
- Ubiquitin is a 76 amino acid protein with a molecular mass of 8.5 kD.
- Distribution
-
Found in almost all tissues of eukaryotic organisms
- Function
- Regulatory protein
- Biology Area
- Cell Biology, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Trafficking and Clearance, Signal Transduction
- Molecular Family
- Autophagosome Markers
- Antigen References
-
1. Hatanaka H, et al. 2009. J. Biol. Chem. 23:15448. (WB) PubMed
2. Sarkari F, et al. 2013. J. Biol. Chem. 23:16975. (WB) PubMed
- Gene ID
- 7316 View all products for this Gene ID
- UniProt
- View information about Ubiquitin on UniProt.org
