HRP anti-Ubiquitin Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 2058	1

Product Details

Verified Reactivity

Human, Mouse, Rat, Drosophila

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Clone P4G7 was raised against denatured bovine ubiquitin and recognizes ubiquitin, polyubiquitin, and ubiquitin-conjugated proteins.

Formulation

This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.

Preparation

The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.

Application

WB - Quality tested
IHC-P - Verified

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 2.5 - 10 ?g per ml.?For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 2.5 - 5.0 ?g/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

P4G7 is equivalent to MAb P4D1. The ubiquitin protein is extremely well conserved and thus the antibody has extensive species cross-reactivity from yeast to human. This antibody was specifically developed to detect ubiquitin and ubiquitin-substrate conjugates by immunoblotting.

Application References (PubMed link indicates BioLegend citation)

Duttler S, et al. 2013. Mol. Cell. 3:379. (WB) PubMed
Wu Y, et al. 2007. Mol. Cell. Biol. 18:6407. (WB) PubMed

RRID

AB_2801179 (BioLegend Cat. No. 838705) AB_2801180 (BioLegend Cat. No. 838706)

Antigen Details

Structure: Ubiquitin is a 76 amino acid protein with a molecular mass of 8.5 kD.
Distribution: Found in almost all tissues of eukaryotic organisms
Function: Regulatory protein
Biology Area: Cell Biology, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Trafficking and Clearance, Signal Transduction
Molecular Family: Autophagosome Markers
Antigen References: 1. Hatanaka H, et al. 2009. J. Biol. Chem. 23:15448. (WB) PubMed
2. Sarkari F, et al. 2013. J. Biol. Chem. 23:16975. (WB) PubMed
Gene ID: 7316 View all products for this Gene ID
UniProt: View information about Ubiquitin on UniProt.org

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