Direct-Blot™ HRP anti-Ubiquitin Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µL	￥ 1414	1

Product Details

Verified Reactivity

All Species

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Denatured glutaraldehyde cross-linked ubiquitin IgG complex

Formulation

This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.

Preparation

The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.

Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:4000. The optimal dilution should be determined by titration for each individual assay of interest.

25 ?l and 100 ?l of Direct-Blot? HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.

Application Notes

This antibody recognizes ubiquitin, polyubiquitin, and ubiquitin-conjugated proteins. This clone is equivalent to Clone P4G7. The ubiquitin protein is extremley well conserved and thus the antibody has extensive species cross-reactivity from yeast to human. This antibody was specifically developed to detect ubiquitin and?ubiquitin-substrate conjugates by immunoblotting. Because the antibody recognizes a denatured epitope, it is known to not work in immunoprecipitation or immunostaining applications.

Application References (PubMed link indicates BioLegend citation)

Magnaudeix A, et al. 2013. Neurobiol Aging. 34:770. PubMed.

RRID

AB_2734516 (BioLegend Cat. No. 646304) AB_2629597 (BioLegend Cat. No. 646303)

Antigen Details

Structure: Consists of 76 amino acids and has a molecular mass of about 8.5 kd. Highly conserved among all eukaryotic species.
Biology Area: Cell Biology, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Trafficking and Clearance, Ubiquitin/Protein Degradation
Molecular Family: Autophagosome Markers
Antigen References: 1. Kahana, et al. 1999. Mol. Cell Biol 19:6608
2. Ciechanover A, et al. 1998. EMBO J 17:7151
3. Xu P, et al. 2008. Analytical Chemistry 80:3438
Gene ID: 6233 View all products for this Gene ID
UniProt: View information about Ubiquitin on UniProt.org

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