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Biotin anti-Ubiquitin Antibody
产品名称:
Biotin anti-Ubiquitin Antibody
产品类别:
抗体
产品编号:
646305
产品应用:
646305
[价格]
规格 价格 库存
25µg ¥ 2058 1

产品详情

Product Details

Verified Reactivity
All Species
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Denatured glutaraldehyde cross-linked ubiquitin IgG complex
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125?- 1.0??g/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody recognizes ubiquitin, polyubiquitin, and ubiquitin-conjugated proteins. This clone is equivalent to Clone P4G7. The ubiquitin protein is extremley well conserved and thus the antibody has extensive species cross-reactivity from yeast to human. This antibody was specifically developed to detect ubiquitin and?ubiquitin-substrate conjugates by immunoblotting. Because the antibody recognizes a denatured epitope, it is known to not work in immunoprecipitation or immunostaining applications.

Application References

(PubMed link indicates BioLegend citation)
  1. Magnaudeix A, et al. 2013. Neurobiol Aging. 34:770. PubMed.
RRID
AB_2888821 (BioLegend Cat. No. 646305) AB_2888821 (BioLegend Cat. No. 646306)

Antigen Details

Structure
Consists of 76 amino acids and has a molecular mass of about 8.5 kd. Highly conserved among all eukaryotic species.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Trafficking and Clearance, Ubiquitin/Protein Degradation
Molecular Family
Autophagosome Markers
Antigen References

1. Kahana, et al. 1999. Mol. Cell Biol 19:6608
2. Ciechanover A, et al. 1998. EMBO J 17:7151
3. Xu P, et al. 2008. Analytical Chemistry 80:3438

Gene ID
6233 View all products for this Gene ID
UniProt
View information about Ubiquitin on UniProt.org

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