PE/Fire™ 810 anti-human CD336 (NKp44) Antibo-上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 2240	1

Product Details

Verified Reactivity

Human

Reported Reactivity

African Green, Baboon, Cynomolgus, Rhesus

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

recombinant human NKp44

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with PE/Fire? 810 under optimal conditions.

Concentration

Lot-specific (please contact technical support for concentration and total ?g amount, or use our Lookup tool if you have a lot number.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?L per million cells in 100 ?L staining volume or 5 ?L per 100 ?L of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* PE/Fire? 810 has a maximum excitation of 488/561 nm and a maximum emission of 810 nm.

Excessive exposure to light, and commonly used fixation, permeabilization buffers can affect PE/Fire? 810 fluorescence signal intensity and spread. Please keep conjugates protected from light exposure. For more information and representative data, visit our Fire Dyes page.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

The p44-8 antibody against human NKp44 has been shown to be useful for flow cytometry, stimulation of human NK cells via NKp44 in a redirected lysis assay, and blocking of NKp44 function in solution. Additional reported applications (for the relevant formats) include: stimulation of human NK cells via NKp44 in a redirected lysis assay, and blocking of NKp44 function in solution^1,2. The LEAF? purified antibody (Endotoxin < 0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 325104).

Application References (PubMed link indicates BioLegend citation)

Sommaggio R, et al. 2012. J. Immunol. 188:2075. (Block)
Hoechst B, et al. 2009. Hepatology. 50:799. (Block) PubMed
Correia DV, et al. 2011. Blood. 118:992. (FC) PubMed

RRID

AB_2927930 (BioLegend Cat. No. 325127)

Antigen Details

Structure: Type I transmembrane protein, member of the natural cytotoxicity receptor family, contains one immunoglobulin-like domain. Approximately 44 kD. Three isoforms produced by alternative splicing.
Distribution: IL-2 activated NK cells and cell lines, some γ/δ T cells activated in vitro
Function: NK cell triggering of cytolysis toward tumor targets and other target cells deficient in MHC class I molecules
Interaction: DAP12
Cell Type: NK cells, T cells
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Cantoni C, et al. 1999. J. Exp. Med. 189:787.
2. Allcock RJN, et al. 2003. Eur. J. Immunol. 33:567.
3. Cantoni C, et al. 2003. Structure. 11:725.
Gene ID: 9436 View all products for this Gene ID
UniProt: View information about CD336 on UniProt.org

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