Purified anti-CD106 (VCAM-1) Antibody (Previously
- 产品名称:
- Purified anti-CD106 (VCAM-1) Antibody (Previously
- 产品类别:
- 抗体
- 产品编号:
- 919801
- 产品应用:
- 919801
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- The P3C4 monoclonal antibody was generated against IL-1β activated human umbilical vein endothelial cells (HUVECs).
- Formulation
- Phosphate-buffered solution (no preservatives or carrier proteins).
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
- Application
-
IHC-F - Quality tested
IP, ICC, FC
- Recommended Usage
Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections.
The optimal working dilution should be determined for each specific assay condition.
? IF: 1:1,000
? IP: 1:1,000
? FC: 1:1,000
? IHC: 1:500 - 1:1000 using an AcuityAdvanced Kit available in the BioLegend Antibody Products web store
Tissue: Frozen sections
Pretreatment: None?
Primary?Incubation: 60 minutes at RT
- Application Notes
This antibody is effective in immunohistochemistry (IHC), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FC), but only on IL-1? activated cells.
IHC Positive Control: Human Ovarian Tumor
This antibody inhibits VCAM-1 binding by a4?1. When used with another monoclonal antibody directed to an alternative epitope this antibody can be used to capture soluble serum VCAM.?
- Application References
(PubMed link indicates BioLegend citation) -
- Kim JA, et al. 1994. Arterioscler Thromb. 14:427.
- RRID
- AB_2565212 (BioLegend Cat. No. 919801)