Ultra-LEAF™ Purified anti-human CD337 (NKp30-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 3780	1

Product Details

Verified Reactivity

Human

Reported Reactivity

African Green, Baboon, Cynomolgus, Rhesus

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Recombinant human NKp30

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
ELISA, Block - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 ?g per million cells in 100 ?l volume or 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: ELISA, stimulation of human NK cells via NKp30 in a redirected lysis assay, and blocking of NKp30 function in solution^1,3,5. The Ultra-LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 325223 & 325224).

Application References (PubMed link indicates BioLegend citation)

Stark S, et al. 2005. J. Immunol. Methods 296:149. (Block)
Mansater I, et al. 2008. J. Immunol. 181:1869. PubMed
Markel G, et al. 2009. PLOS One 4:e5597. (Block)
Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
Byrd A, et al. 2007. PLoS One. 2:e1339. (Block)

Product Citations

Myers JA, et al. 2022. JCI Insight. :. PubMed

RRID

AB_2814182 (BioLegend Cat. No. 325223) AB_2814183 (BioLegend Cat. No. 325224)

Antigen Details

Structure: Type I transmembrane protein, member of the natural cytotoxicity receptor family, contains one immunoglobulin-like domain. Approximately 44 kD. Six isoforms produced by alternative splicing.
Distribution: IL-2 activated NK cells and NK cell lines, some γ/δ T cells activated in vitro
Function: NK cell triggering of cytolysis toward tumor targets and other target cells deficient in MHC class I molecules
Interaction: CD59, TCRζ
Cell Type: NK cells, T cells
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Pende D, et al. 1999. J. Exp. Med. 190:1505.
Gene ID: 259197 View all products for this Gene ID
UniProt: View information about CD337 on UniProt.org

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