Brilliant Violet 605™ anti-human CD337 (NKp3-上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 2856	1

Product Details

Verified Reactivity

Human

Reported Reactivity

African Green, Baboon, Cynomolgus, Rhesus

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Recombinant human NKp30

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605? under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?L per million cells in 100 ?L staining volume or 5 ?L per 100 ?L of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 605? excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605? is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application Notes

Additional reported applications (for the relevant formats) include: ELISA, stimulation of human NK cells via NKp30 in a redirected lysis assay, and blocking of NKp30 function in solution^1,3,5. The Ultra-LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 325223 & 325224).

Application References (PubMed link indicates BioLegend citation)

Stark S, et al. 2005. J. Immunol. Methods 296:149. (Block)
Mansater I, et al. 2008. J. Immunol. 181:1869. PubMed
Markel G, et al. 2009. PLOS One 4:e5597. (Block)
Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
Byrd A, et al. 2007. PLoS One. 2:e1339. (Block)

RRID

AB_2892388 (BioLegend Cat. No. 325233) AB_2892388 (BioLegend Cat. No. 325234)

Antigen Details

Structure: Type I transmembrane protein, member of the natural cytotoxicity receptor family, contains one immunoglobulin-like domain. Approximately 44 kD. Six isoforms produced by alternative splicing.
Distribution: IL-2 activated NK cells and NK cell lines, some γ/δ T cells activated in vitro
Function: NK cell triggering of cytolysis toward tumor targets and other target cells deficient in MHC class I molecules
Interaction: CD59, TCRζ
Cell Type: NK cells, T cells
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Pende D, et al. 1999. J. Exp. Med. 190:1505.
Gene ID: 259197 View all products for this Gene ID
UniProt: View information about CD337 on UniProt.org

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