Anti-CD31 (PECAM-1) Antibody (Previously Covance c
- 产品名称:
- Anti-CD31 (PECAM-1) Antibody (Previously Covance c
- 产品类别:
- 抗体
- 产品编号:
- 910003
- 产品应用:
- 910003
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- The P2B1 monoclonal antibody was generated against IL-1β activated human umbilical vein endothelial cells (HUVECs).
- Preparation
- Ascites
- Concentration
- The concentration is not quantified as this product is sold as undiluted crude mouse ascites fluid. The concentration might vary from lot-to-lot and an estimated concentration would be 1-3 mg/ml.
- Storage & Handling
- Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C.
- Application
-
IHC-F - Quality tested
ICC, WB, IP - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a dilution range of 1:500 - 1:1000 is suggested.?For immunocytochemistry, a range of 1:250 - 1:500 is recommended. For Western blotting, the suggested use of this reagent is 1:1000 dilution. For immunoprecipitation, the suggested use of this reagent is 1:1000 dilution. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This antibody is effective in immunoblotting, immunocytochemistry (ICC), immunoprecipitation (IP) and immunohistochemistry (IHC).
*Predicted MW = 83 kD
Positive Control: human kidney
- Application References
(PubMed link indicates BioLegend citation) -
- Brooks PC, et al. 1995. J Clin Invest. 96:1815. (IF)
- Product Citations
-
- Taha A, et al. 2020. J Cell Physiol. 7094:235. PubMed
- Zhai X, et al. 2018. Oncol Lett. 15:1893. PubMed
- RRID
- AB_2565394 (BioLegend Cat. No. 910003)