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PerCP/Cyanine5.5 anti-mouse I-A/I-E Antibody
产品名称：
PerCP/Cyanine5.5 anti-mouse I-A/I-E Antibody
产品类别：
抗体
产品编号：
107626
产品应用：
107626
[价格]
规格 价格 库存
100µg ￥ 4522 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Activated C57BL/6 mouse spleen cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.06 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek MHC class II alloantigens from mice carrying H-2p,r,q,b,d,u haplotypes. Clone M5/114.15.2 however does not react wtih I-Af, I-Ak, or I-As MHC class II alloantigens.1

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of frozen sections2,3,6, in vitro and in vivo blocking of antigen presentation or ligand binding4-7, and spatial biology (IBEX)17,18. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 107655 & 107656).

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Bhattacharya A, et al. 1981. J. Immunol. 127:2488. (IP)
  2. Viville S, et al. 1993. Cell 72:635. (IHC)
  3. Nelson AJ, et al. 1993. J. Immunol. 151:2453. (IHC)
  4. Shi Y, et al. 1998. J. Exp. Med. 187:367. (Block)
  5. Yamashita I, et al. 1993. Int. Immunol. 5:1139.
  6. Guo M, et al. 1995. Zygote 3:65. (IHC)
  7. Kim A, et al. 2004. Exp. Mol. Med. 36:428. (Block)
  8. Luckashenak NA, et al. 2006. J. Immunol. 177:5177.
  9. Venanzi ES, et al. 2007. J. Immunol. 179:5693.
  10. Christensen SR, et al. 2006. Immunity 25:417. PubMed
  11. Matte-Martone C, et al. 2008. Blood 111:3884. PubMed
  12. De Pascalis R, et al. 2008. Infect. Immun. 76:4311. PubMed
  13. Kuns RD, et al. 2009. Blood 113:5999. PubMed
  14. Sabatino JJ, et al. 2011. J. Exp. Med. 208:81. PubMed
  15. Draber P, et al. 2011. Mol Cell Biol. 22:4550. PubMed
  16. Fu H, et al. 2014. Nat Commun. 5:3436. PubMed
  17. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
  18. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
Product Citations
  1. Saha D et al. 2017. Cancer cell. 32(2):253-267 . PubMed
  2. Herz J, et al. 2015. J Exp Med. 212: 1153 - 1169. PubMed
  3. Ortiz G, et al. 2020. Front Immunol. 11:1713. PubMed
  4. Nakamura M, et al. 2015. J Immunol. 194:243. PubMed
  5. Gon?alves NP, et al. 2017. J Neuroinflammation. 14:115. PubMed
  6. Matsumoto R, et al. 2021. Front Pharmacol. 12:715752. PubMed
  7. Kong D, et al. 2022. J Heart Lung Transplant. Online ahead of print. PubMed
  8. Clement M, et al. 2016. PLoS Pathog. 12:e1006050. PubMed
  9. Chow MT et al. 2019. Immunity. 50(6):1498-1512 . PubMed
  10. Gounder AP, et al. 2018. PLoS Pathog. 14:e1007001. PubMed
  11. Rao E, et al. 2021. Sci Immunol. 6:. PubMed
  12. Volkov A, et al. 2013. PLoS One. 8:60565. PubMed
  13. Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed
  14. Li S, et al. 2021. Ann Transl Med. 9:1644. PubMed
  15. Perner C, et al. 2020. Immunity. 53(5):1063-1077.e7. PubMed
  16. Zhuang Z, et al. 2021. J Exp Med. 218:00:00. PubMed
  17. Sharma S, et al. 2015. J Immunol. 194:5529. PubMed
  18. Sharma PK, et al. 2018. Cancer Gene Ther. 25:27. PubMed
  19. Pello O, et al. 2012. PLoS One. 7:e45399. PubMed
  20. Vijay R, et al. 2015. J Exp Med. 212: 1851 - 1868. PubMed
  21. Terashima Y, et al. 2020. Nat Commun. 11:609. PubMed
  22. Liang Z, et al. 2020. Pain. 161:2089. PubMed
  23. Fantauzzi MF, et al. 2021. ERJ Open Res. 7: . PubMed
  24. Funk KE, et al. 2019. J Neuroinflammation. 16:22. PubMed
  25. Donati Y, et al. 2020. Am J Physiol Lung Cell Mol Physiol. L619:318. PubMed
  26. Grzelak A, et al. 2018. Int J Mol Sci. 19:. PubMed
  27. Codina A, et al. 2019. Cell Syst. 8:136. PubMed
  28. McFarlane AJ, et al. 2017. J Allergy Clin Immunol. 140:1068. PubMed
  29. Wang J, et al. 2021. Nat Commun. 12:6198. PubMed
  30. Dobosz E, et al. 2021. Dis Model Mech. :14. PubMed
  31. Xue N, et al. 2017. Sci Rep. 7:39011. PubMed
  32. Bartmann J, et al. 2016. Int Immunol. 28: 473 - 487. PubMed
  33. Ji DX, et al. 2019. Nat Microbiol. 1.644444444. PubMed
  34. Yang Y, et al. 2021. Sci Adv. 7:eabf6290. PubMed
  35. Zhang Q, et al. 2022. Nat Commun. 13:2406. PubMed
  36. Nishida J, et al. 2022. STAR Protoc. 3:101306. PubMed
  37. Fidalgo MF, et al. 2022. Life Sci Alliance. 5:. PubMed
  38. Rozanski C, et al. 2015. J Immunol. 194:4717. PubMed
  39. Movahedi K, et al. 2012. Cancer Res. 74:4165. PubMed
  40. Yu X, et al. 2019. Nat Commun. 10:574. PubMed
  41. Georgoudaki A, et al. 2016. Cell Rep. 15: 2000-2011. PubMed
  42. Melo-Silva CR, et al. 2021. PLOS Pathogens. 17(5):e1009593. PubMed
  43. Fu Y, et al. 2021. Front Cell Dev Biol. 9:689727. PubMed
  44. Yi H, et al. 2021. Front Immunol. 12:719189. PubMed
  45. Clement CC, et al. 2021. Immunity. 54:721. PubMed
  46. Bent EH, et al. 2021. Nat Commun. 12:6218. PubMed
  47. Lu X, et al. 2019. Circ Res. 125:1055. PubMed
  48. Zhang MS, et al. 2022. Nat Commun. 13:954. PubMed
  49. Walker MT, et al. 2021. Front. Allergy. 2:. PubMed
  50. Mairhofer D, et al. 2015. J Invest Dermatol. 135: 2785-93. PubMed
  51. Bell CR, et al. 2022. Nat Commun. 13:2063. PubMed
  52. Alhudaithi SS, et al. 2020. Mol Pharm. 17:4691. PubMed
  53. Kiss M, et al. 2020. Cancer Immunol Res. 9:309. PubMed
  54. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
  55. Aguilar-Pimentel A, et al. 2017. PLoS One. 12(6):e0178563. PubMed
  56. Hildreth AD, et al. 2020. STAR Protoc. 1:100113. PubMed
  57. Lau P, et al. 2022. Cell Mol Immunol. :. PubMed
  58. Stack G, et al. 2015. PLoS Pathog. 11:1004641. PubMed
  59. Lai NY, et al. 2020. Cell. 180:33:00. PubMed
  60. Golabek A, et al. 2021. Exp Ther Med. 21:482. PubMed
  61. Chen R, et al. 2021. Front Oncol. 11:743050. PubMed
  62. Li J, et al. 2021. eLife. 10:00. PubMed
  63. Chng S, et al. 2016. Sci Rep. 6: 23820. PubMed
  64. Andreata F, et al. 2021. STAR Protoc. 2:100831. PubMed
  65. Shi GN, et al. 2021. Ther Adv Med Oncol. 13:1758835920987056. PubMed
RRID
AB_2191072 (BioLegend Cat. No. 107625) AB_2191071 (BioLegend Cat. No. 107626)

Antigen Details

Structure
MHC class II
Distribution

B cell and activated T cells, APCs of the H-2b,d,q,r bearing mice

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
Antigen-presenting cells, B cells, Dendritic cells, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.

Gene ID
14961 View all products for this Gene ID 14969 View all products for this Gene ID
UniProt
View information about I-A/I-E on UniProt.org

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