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首页 > 抗体蛋白 > 抗体
Brilliant Violet 421™ anti-mouse I-A/I-E Ant
产品名称：
Brilliant Violet 421™ anti-mouse I-A/I-E Ant
产品类别：
抗体
产品编号：
107632
产品应用：
107632
[价格]
规格 价格 库存
50µg ￥ 3318 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Activated C57BL/6 mouse spleen cells
Formulation
?l size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
?g size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421? under optimal conditions.
Concentration
?g size: 0.2 mg/mL
?L size: lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F - Verified

SB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the ?l size, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood. For flow cytometric staining using the ?g size, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421? excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421? is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek MHC class II alloantigens from mice carrying H-2p,r,q,b,d,u haplotypes. Clone M5/114.15.2 however does not react wtih I-Af, I-Ak, or I-As MHC class II alloantigens.1

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of frozen sections2,3,6, in vitro and in vivo blocking of antigen presentation or ligand binding4-7, and spatial biology (IBEX)17,18. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 107655 & 107656).

Additional Product Notes

Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).

Application References

(PubMed link indicates BioLegend citation)
  1. Bhattacharya A, et al. 1981. J. Immunol. 127:2488. (IP)
  2. Viville S, et al. 1993. Cell 72:635. (IHC)
  3. Nelson AJ, et al. 1993. J. Immunol. 151:2453. (IHC)
  4. Shi Y, et al. 1998. J. Exp. Med. 187:367. (Block)
  5. Yamashita I, et al. 1993. Int. Immunol. 5:1139.
  6. Guo M, et al. 1995. Zygote 3:65. (IHC)
  7. Kim A, et al. 2004. Exp. Mol. Med. 36:428. (Block)
  8. Luckashenak NA, et al. 2006. J. Immunol. 177:5177.
  9. Venanzi ES, et al. 2007. J. Immunol. 179:5693.
  10. Christensen SR, et al. 2006. Immunity 25:417. PubMed
  11. Matte-Martone C, et al. 2008. Blood 111:3884. PubMed
  12. De Pascalis R, et al. 2008. Infect. Immun. 76:4311. PubMed
  13. Kuns RD, et al. 2009. Blood 113:5999. PubMed
  14. Sabatino JJ, et al. 2011. J. Exp. Med. 208:81. PubMed
  15. Draber P, et al. 2011. Mol Cell Biol. 22:4550. PubMed
  16. Fu H, et al. 2014. Nat Commun. 5:3436. PubMed
  17. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
  18. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
Product Citations
  1. Sagnella SM, et al. 2020. Cancer Cell. 37(3):354-370. PubMed
  2. Zhao Z, et al. 2021. Nat Commun. 12:4355. PubMed
  3. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  4. Friess MC, et al. 2022. Cell Rep. 38:110334. PubMed
  5. Iwamoto H et al. 2018. Cell metabolism. 28(1):104-117 . PubMed
  6. Song Q, et al. 2022. Front Immunol. 13:920232. PubMed
  7. Postat J et al. 2018. Immunity. 49(4):654-665 . PubMed
  8. Sepe JJ, et al. 2022. JACC Basic Transl Sci. 7:915. PubMed
  9. Schmidleithner L et al. 2019. Immunity. 50(5):1232-1248 . PubMed
  10. Potluri HK, et al. 2022. J Immunother Cancer. 10:. PubMed
  11. Ostendorf BN, et al. 2020. Nat Med. 26:1048. PubMed
  12. Perner C, et al. 2020. Immunity. 53(5):1063-1077.e7. PubMed
  13. Mariani SA, et al. 2019. Immunity. 50:1439. PubMed
  14. Voisin M, et al. 2021. Commun Biol. 4:420. PubMed
  15. Wang F, et al. 2021. Cell Mol Gastroenterol Hepatol. 13:257. PubMed
  16. Salei N, et al. 2020. J Am Soc Nephrol. 31:257. PubMed
  17. Mehta AK, et al. 2021. Nat Cancer. 2:66. PubMed
  18. Ferreira R, et al. 2016. J Infect Dis. 213: 669 - 673. PubMed
  19. Reed J, et al. 2020. Bio Protoc. 10:e3607. PubMed
  20. Tavazoie MF, et al. 2018. Cell. 172:825. PubMed
  21. Roberts LM, et al. 2021. iScience. 24:103025. PubMed
  22. Li Y, et al. 2021. Sci Transl Med. 13:. PubMed
  23. Panea C, et al. 2021. Commun Biol. 4:913. PubMed
  24. Wang Y, et al. 2020. Cell Death Dis. 1.169444444. PubMed
  25. Lim CX, et al. 2020. Cell Rep. 3793:30. PubMed
  26. Grauel AL, et al. 2020. Nat Commun. 4.84375. PubMed
  27. Luo J et al. 2018. Immunity. 49(1):107-119 . PubMed
  28. Wang YT, et al. 2020. Nat Microbiol. 0.397222222. PubMed
  29. Alam Z, et al. 2020. Cell Rep. 107825:31. PubMed
  30. Zhang Q, et al. 2021. Front Cell Dev Biol. 9:655552. PubMed
  31. Nair S, et al. 2021. JCI Insight. 6:. PubMed
  32. Taylor D, et al. 2022. Front Immunol. 13:936129. PubMed
  33. Nabet BY et al. 2017. Cell. 170(2):352-366 . PubMed
  34. Benci JL et al. 2019. Cell. 178(4):933-948 . PubMed
  35. Nenasheva T, et al. 2017. PLoS One. 12(6):e0178983. PubMed
  36. Matsuda T, et al. 2022. iScience. 25:105324. PubMed
  37. Dong X, et al. 2022. Front Immunol. 13:896472. PubMed
  38. Takahashi F, et al. 2022. iScience. 25:104278. PubMed
  39. Dotsey E, et al. 2017. Sci Rep. 7:42584. PubMed
  40. Barbet G, et al. 2018. Immunity. 48:584. PubMed
  41. Lai SM et al. 2018. Cell reports. 25(11):3099-3109 . PubMed
  42. Zhang Q, et al. 2022. Commun Biol. 5:544. PubMed
  43. Xiao Y, et al. 2021. Cell. 184:6037. PubMed
  44. Kiss M, et al. 2020. Cancer Immunol Res. 9:309. PubMed
  45. Yu H, et al. 2021. Cell Death Dis. 13:1. PubMed
  46. Pantelidou C, et al. 2022. NPJ Breast Cancer. 8:102. PubMed
  47. Xu L, et al. 2022. Nat Commun. 13:6881. PubMed
  48. Sheng J, et al. 2021. eLife. 10:00. PubMed
  49. Kunishita Y, et al. 2020. Front Immunol. 11:98. PubMed
  50. Gu AQ, et al. 2019. J Cell Mol Med. 23(8):5403. PubMed
  51. Liang Z, et al. 2017. Autophagy. 14:505. PubMed
  52. Hirota K et al. 2018. Immunity. 48(6):1220-1232 . PubMed
  53. Mellal K, et al. 2019. Sci Rep. 9:12903. PubMed
  54. Mincham KT, et al. 2021. Front Immunol. 11:601494. PubMed
  55. Luo ZW, et al. 2021. Int J Nanomedicine. 16:2949. PubMed
  56. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
  57. Sadiq BA, et al. 2020. Current Protocols in Immunology. 131(1):e115. PubMed
  58. Trivedi S, et al. 2020. Elife. 9:00. PubMed
  59. Liang Z, et al. 2022. iScience. 25:105233. PubMed
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RRID
AB_10900075 (BioLegend Cat. No. 107631) AB_2650896 (BioLegend Cat. No. 107632)

Antigen Details

Structure
MHC class II
Distribution

B cell and activated T cells, APCs of the H-2b,d,q,r bearing mice

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
Antigen-presenting cells, B cells, Dendritic cells, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.

Gene ID
14961 View all products for this Gene ID 14969 View all products for this Gene ID
UniProt
View information about I-A/I-E on UniProt.org

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