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Alexa Fluor® 647 anti-mouse CD31 Antibody
产品名称：
Alexa Fluor® 647 anti-mouse CD31 Antibody
产品类别：
抗体
产品编号：
102515
产品应用：
102515
[价格]
规格 价格 库存
25µg ￥ 1414 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Polyoma middle T transformed EC line tEnd.1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
3D IHC - Verified

SB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?L?volume.?For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 ?g/mL?is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.8 Additional reported applications (in the relevant formats) include: immunoprecipitation1, in vitro and in vivo blocking of CD31-mediated cell-cell interactions1-4, immunohistochemical staining1,5,6 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and spatial biology (IBEX)12,13.
Special Note: The antibody works well on acetone-fixed frozen sections as well as Zinc-fixed paraffin-embedded sections. It sometime works on formalin-fixed and paraformaldehyde-fixed paraffin-embedded tissue sections but inconsistent results have been reported. This antibody is not recommended for formalin-fixed paraffin-embedded sections or for Western blot analysis. The Ultra-LEAF? Purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 102529 and 102530).

Application References

(PubMed link indicates BioLegend citation)
  1. Vecchi A, et al. 1994. Eur. J. Cell Biol. 63:247. (IP, IHC, Block)
  2. Christofidou-Solomidou M, et al. 1997. J. Immunol. 158:4872. (Block)
  3. DeLisser HM, et al. 1997. Am. J. Pathol. 151:671. (Block)
  4. Rosenblum WI, et al. 1994. Am. J. Pathol. 145:33. (Block)
  5. Baldwin HS, et al. 1994. Development 120:2539. (IHC)
  6. Voswinckel R, et al. 2003. Circ. Res. 93:372. (IHC)
  7. Leung VW, et al. 2009. Am J. Pathol. 175:1757. PubMed
  8. Chacko AM, et al. 2012. PLoS One 7:e34958.
  9. Giacomini C, et al. 2014. Exp Eye Res. 18:1. PubMed
  10. Morita R, et al. 2015. PNAS. 112:160. PubMed
  11. Ito A, et al. 2015. Brain Res. 1594:310. PubMed
  12. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
  13. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
Product Citations
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  5. Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed
  6. Merz SF, et al. 2019. Nat Commun. 10:2312. PubMed
  7. Mason HD, et al. 2021. JCI Insight. 6:e149229. PubMed
  8. G?tz P, et al. 2022. Int J Mol Sci. 23:. PubMed
  9. Kumaraswami K, et al. 2020. Int J Mol Sci. 21:00. PubMed
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  12. Evren E, et al. 2020. Immunity. 54(2):259-275.e7. PubMed
  13. Jiang YH, et al. 2020. J Mol Cell Cardiol. 138:115. PubMed
  14. Perry H, et al. 2016. J Am Soc Nephrol. 10.1681/ASN.2015080922. PubMed
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  29. Shannon JP, et al. 2021. STAR Protoc. 2:100790. PubMed
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  31. Pinho S et al. 2018. Developmental cell. 44(5):634-641 . PubMed
  32. Lustgarten Guahmich N, et al. 2020. Angiogenesis. 23:443. PubMed
  33. Kübler M, et al. 2021. Int J Mol Sci. 22:. PubMed
  34. Stacy A, et al. 2021. Cell. 184(3):615-627.e17. PubMed
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  36. Alexandre YO, et al. 2020. Cell Reports. 33(13):108567. PubMed
  37. Cheng HW, et al. 2022. Nat Commun. 13:2027. PubMed
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  44. Hsu KS, et al. 2022. Nat Commun. 13:7078. PubMed
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  49. Rodriguez AB, et al. 2022. Front Immunol. 13:1009306. PubMed
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  51. Gao X, et al. 2021. Nature. 589:591. PubMed
  52. Baron CS, et al. 2018. Nat Commun. 9:2517. PubMed
  53. Shimba A et al. 2018. Immunity. 48(2):286-298 . PubMed
  54. Xu M, et al. 2020. Cell. 178(6):1478-1492.e20.. PubMed
  55. Hoyer FF, et al. 2020. Immunity. 51(5):899-914.e7.. PubMed
  56. Klingberg A, et al. 2016. J Am Soc Nephrol. 10.1681/ASN.2016020232. PubMed
  57. Courties G, et al. 2019. Circ Res. 124:1372. PubMed
  58. Winter C, et al. 2018. Cell Metab. 28:175. PubMed
  59. Lasch M, et al. 2020. Front Physiol. 11:576736. PubMed
  60. Sakamoto K, et al. 2022. STAR Protoc. 3:101052. PubMed
  61. Asada N, et al. 2017. Nat Cell Biol. 19:214-223. PubMed
  62. Lee EKS, et al. 2018. Cell Host Microbe. 23:121. PubMed
  63. Severe N et al. 2019. Cell Stem Cell. 25(4):570-583 . PubMed
  64. Stanwick M, et al. 2022. Front Cell Dev Biol. 10:834815. PubMed
  65. Kikuchi H, et al. 2020. Cancer Res. 80:2996. PubMed
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RRID
AB_2161030 (BioLegend Cat. No. 102515) AB_2161029 (BioLegend Cat. No. 102516)

Antigen Details

Structure
Ig superfamily, 130-140 kD
Distribution

Endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, T and B cell subsets

Function
Adhesion
Ligand/Receptor
CD38, αV/β3 integrin
Cell Type
B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Monocytes, Neutrophils, Platelets, T cells
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. DeLisser HM, et al. 1994. Immunol. Today 15:490.
3. Newman PJ, et al. 1990. Science 247:1219.

Gene ID
18613 View all products for this Gene ID
UniProt
View information about CD31 on UniProt.org

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