Purified anti-human CD271 (NGFR) Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1246	1

Product Details

Verified Reactivity

Human

Reported Reactivity

African Green, Baboon, Cynomolgus, Rhesus

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

WM245 melanoma cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

FC - Quality tested
IP, WB, IHC-P, IHC-F - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications for the relevant formats include: immunoprecipitation¹, Western blotting⁴, immunohistochemical staining of frozen or paraffin embedded tissue sections^1,2, and blocking of NGF binding¹.

Application References (PubMed link indicates BioLegend citation)

Ross A, et al. 1984. P. Natl. Acad. Sci. USA 81:6681. (FC, IP, Block, IHC)
Cattoretti G, et al. 1993. Blood 81:1726. (IHC)
Kamke MR, et al. 2005. Hear Res. 206:89.
Baker D, et al. 1989. Cancer Res. 49:4142-4146. (FC, WB)

Product Citations

Torre E et al. 2018. Cell systems. 6(2):171-179 . PubMed
Krovi SH, et al. 2019. Proc Natl Acad Sci U S A. 116:22252. PubMed
Chen Y, et al. 2020. Blood Adv. 4:5257. PubMed
Pilbauerova N, et al. 2021. Int J Mol Sci. 22:. PubMed
Morsing M, et al. 2020. Breast Cancer Res. 0.9875. PubMed
Tey SR, et al. 2022. Stem Cells Int. 2022:2735414. PubMed
Li H, et al. 2020. Haematologica. 105:1206. PubMed

RRID

AB_1937276 (BioLegend Cat. No. 345101) AB_1937275 (BioLegend Cat. No. 345102)

Antigen Details

Structure: Type I transmembranal protein with a MW of 75 kD. The extracellular portion contains four TNFR-Cys repeats that form the NGF binding domain; the intracellular portion contains a death domain.
Distribution: Expressed by many cell types including neurons, Schwann cells, mesenchymal stem/stromal cells, follicular dendritic cells, melanocytes.
Function: Apoptosis, differentiation, neurogenesis.
Interaction: TRAF2, TRAF4, TRAF6, PTPN13, RANBP9.
Ligand/Receptor: Nerve growth factor (NGF), Brain-derived neurotrophic factor (BDNF), Neurotrophin 3. (NTF3) , Neurotrophin 4 (NTF4)
Bioactivity: Apoptosis, differentiation, neurogenesis.
Cell Type: Dendritic cells, Mesenchymal cells, Mesenchymal Stem Cells, Neurons
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, Postsynaptic proteins
Antigen References: 1. Friedman MJ, et al. 2009. J. Biol. Chem. 284:27944.
2. Rock JR, et al. 2009. Proc Natl Acad Sci USA. 106:12771.
3. Kidd SK, et al. 2008. Brain Res.1195:113.
4. Peters EM, et al. 2007. Am J Pathol. 171:1872.
5. Jansen P, et al. 2007. Nat Neurosci. 10:1449.
Gene ID: 4804 View all products for this Gene ID
UniProt: View information about CD271 on UniProt.org

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