Biotin anti-human CD271 (NGFR) Antibody
- 产品名称：
- Biotin anti-human CD271 (NGFR) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 345122
- 产品应用：
- 345122
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Baboon, Cynomolgus, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- WM245 melanoma cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications for the relevant formats include: immunoprecipitation1, Western blotting4, immunohistochemical staining of frozen or paraffin embedded tissue sections1,2, and blocking of NGF binding1.
- Application References
(PubMed link indicates BioLegend citation) -
- Ross A, et al. 1984. P. Natl. Acad. Sci. USA 81:6681. (FC, IP, Block, IHC)
- Cattoretti G, et al. 1993. Blood 81:1726. (IHC)
- Kamke MR, et al. 2005. Hear Res. 206:89.
- Baker D, et al. 1989. Cancer Res. 49:4142-4146. (FC, WB)
- Product Citations
-
- Hinz S, et al. 2021. iScience. 24:103026. PubMed
- RRID
- AB_2810548 (BioLegend Cat. No. 345122)
- Structure
- Type I transmembranal protein with a MW of 75 kD. The extracellular portion contains four TNFR-Cys repeats that form the NGF binding domain; the intracellular portion contains a death domain.
- Distribution
-
Expressed by many cell types including neurons, Schwann cells, mesenchymal stem/stromal cells, follicular dendritic cells, melanocytes.
- Function
- Apoptosis, differentiation, neurogenesis.
- Interaction
- TRAF2, TRAF4, TRAF6, PTPN13, RANBP9.
- Ligand/Receptor
- Nerve growth factor (NGF), Brain-derived neurotrophic factor (BDNF), Neurotrophin 3. (NTF3) , Neurotrophin 4 (NTF4)
- Bioactivity
- Apoptosis, differentiation, neurogenesis.
- Cell Type
- Dendritic cells, Mesenchymal cells, Mesenchymal Stem Cells, Neurons
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroscience, Stem Cells, Synaptic Biology
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors, Postsynaptic proteins
- Antigen References
-
1. Friedman MJ, et al. 2009. J. Biol. Chem. 284:27944.
2. Rock JR, et al. 2009. Proc Natl Acad Sci USA. 106:12771.
3. Kidd SK, et al. 2008. Brain Res.1195:113.
4. Peters EM, et al. 2007. Am J Pathol. 171:1872.
5. Jansen P, et al. 2007. Nat Neurosci. 10:1449.
- Gene ID
- 4804 View all products for this Gene ID
- UniProt
- View information about CD271 on UniProt.org
