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Purified anti-human CD3 Antibody
产品名称：
Purified anti-human CD3 Antibody
产品类别：
抗体
产品编号：
300302
产品应用：
300302
[价格]
规格 价格 库存
100µg ￥ 700 1

产品详情

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP, IHC-F, Activ - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections, immunoprecipitation, and activation of T lymphocytes4-7. The HIT3a antibody is able to stimulate T cell activation. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 300314). For highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 300332) with a lower endotoxin limit than standard LEAF? purified antibodies (Endotoxin <0.01 EU/?g).

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
  3. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
  4. Sedelies KA, et al. 2004. J. Biol. Chem. 279:26581. (Activ)
  5. Rivollier A, et al. 2004. Blood 104:4029. (Activ)
  6. Scharschmidt E, et al. 2004. Mol. Cell Biol. 24:3860. (Activ)
  7. Smeltz RB. 2007. J. Immunol. 178:4786. (Activ)
Product Citations
  1. Lin GL, et al. 2018. Acta Neuropathol Commun. 9:617. PubMed
  2. Kaushal A, et al. 2021. Blood Cancer Discov. 2:600. PubMed
  3. Galaski J, et al. 2021. Front Cell Infect Microbiol. 11:692544. PubMed
  4. Yang W, et al. 2020. Nat Commun. 3.553472222. PubMed
  5. Kim H, et al. 2021. Int J Mol Sci. 22:. PubMed
  6. Gao H, et al. 2021. J Virol. :. PubMed
  7. Patel AA, et al. 2017. J Exp Med. 214:1913. PubMed
  8. Willimsky G, et al. 2021. Elife. 10:. PubMed
  9. Chakhtoura M, et al. 2021. PLoS Pathog. 17:e1009732. PubMed
  10. Vereertbrugghen A, et al. 2021. Front Oncol. 11:598319. PubMed
  11. Zhang Y, et al. 2022. Exp Hematol Oncol. 11:15. PubMed
  12. Kondo H, et al. 2022. Front Immunol. 13:836923. PubMed
  13. Liu F, et al. 2020. J Immunother Cancer. 8:. PubMed
  14. Jin H, et al. 2019. MBio. 10:e01769-19. PubMed
  15. Kilpinen L, et al. 2013. J Lipid Res. 54:622. PubMed
  16. Cobb DA, et al. 2022. J Immunother Cancer. 10:. PubMed
  17. Bruno TC, et al. 2017. Cancer Immunol Res. 0.831944444. PubMed
  18. Cosgrove PR, et al. 2021. Pediatr Res. . PubMed
  19. Cai J, et al. 2021. eLife. 10:00. PubMed
  20. Liu Y, et al. 2022. Micromachines (Basel). 13:. PubMed
  21. Zhao J, et al. 2017. Sci Immunol. 2:eaan5400. PubMed
  22. Bonavia AS, et al. 2022. Front Immunol. 13:940030. PubMed
  23. Newell KL, et al. 2021. PLoS One. 16:e0244855. PubMed
  24. Vlkova M, et al. 2019. J Immunol. 202:93. PubMed
  25. Sheng WY, et al. 2006. FEBS Lett. 580:6819. PubMed
  26. De Maeyer RPH, et al. 2020. Nat Immunol. 21:615. PubMed
  27. Mazer M, et al. 2019. J Immunol. 203:2088. PubMed
  28. Jones N, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01516. PubMed
  29. Bantug GR, et al. 2018. Immunity. 48:542. PubMed
  30. Ren X, et al. 2021. Lab Chip. 21:1527. PubMed
  31. Ravell JC, et al. 2020. J Clin Invest. 130:507. PubMed
  32. Ainai A, et al. 2020. Microbiol Immunol. 64:313. PubMed
RRID
AB_314037 (BioLegend Cat. No. 300301) AB_314038 (BioLegend Cat. No. 300302)

Antigen Details

Structure
Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution

Mature T and NK-T cells, thymocyte differentiation

Function
Antigen recognition, signal transduction, T cell activation
Ligand/Receptor
Peptide antigen bound to MHC
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.

Gene ID
916 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

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