Alexa Fluor® 700 anti-human CD42b Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 1974	1

Product Details

Verified Reactivity

Human

Reported Reactivity

Chimpanzee

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 700 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

* Alexa Fluor? 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor? 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Excitation Laser

Red Laser (633 nm)

Application Notes

Clone HIP1 recognizes an epitope within the N-terminal region of the GPIba chain⁵. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections, Westerm blotting, and inhibition of platelet aggregation². The Ultra LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 303939).

Application References (PubMed link indicates BioLegend citation)

Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
Takahashi R, et al. 1999. Blood. 93:1951.
Saggu G, et al. 2013. J. Immunol. 190:6457. PubMed
Meyer Dos Santos S, et al. 2011. Blood. 117:4999. (Block) PubMed
Vettore S, et al. 2008. Haematologica. 93:1743.

RRID

AB_2715928 (BioLegend Cat. No. 303927) AB_2715929 (BioLegend Cat. No. 303928)

Antigen Details

Structure: Mucin, leucine-rich repeat family, 145 kD
Distribution: Platelets, megakaryocytes
Function: Complex with CD42a, c and d, platelet adhesion and activation
Ligand/Receptor: Von Willebrand factor, thrombin
Cell Type: Megakaryocytes, Platelets
Biology Area: Cell Adhesion, Cell Biology, Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Clemetson K, et al. 1982. J. Clin. Invest. 70:304.
2. Fox J, et al. 1988. J. Biol. Chem. 263:4882.
3. Kuijpers R, et al. 1992 Blood 79:283.
Gene ID: 2812 View all products for this Gene ID
UniProt: View information about CD42b on UniProt.org

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