Ultra-LEAF™ Purified anti-human CD147 Antibo-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 3780	1

Product Details

Verified Reactivity

Human

Reported Reactivity

Chimpanzee, Baboon, Capuchin Monkey

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Human PBMCs

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/?g of the protein (< 0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
WB, IHC, Block - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 ?g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: inhibition of T cell activation², immunohistochemical staining^1,3 of frozen tissue sections and formalin-fixed paraffin-embedded tissue sections, and Western blotting¹. The Ultra-LEAF? Purified antibody (Endotoxin <0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 306221 and 306222).

Application References (PubMed link indicates BioLegend citation)

Menashi S, et al. 2003. Cancer Res. 63:7575. (WB IHC)
Woodhead VE, et al. 2000. Int. Immunol. 12:1051. (Block)
Reimers N, et al. 2004. Clin. Cancer Res. 10:3422. (IHC)

Product Citations

Avolio E, et al. 2021. Clin Sci (Lond). Online ahead of print. PubMed

RRID

AB_2860815 (BioLegend Cat. No. 306221) AB_2860816 (BioLegend Cat. No. 306222)

Antigen Details

Structure: Ig superfamily, type I transmembrane glycoprotein, 55-65 kD
Distribution: Leukocytes, erythrocytes, platelets, endothelial cells
Function: Adhesion
Cell Type: Endothelial cells, Erythrocytes, Leukocytes, Platelets
Biology Area: Cell Adhesion, Cell Biology, Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Biswas C, et al. 1995. Cancer Res. 55:434.
2. Fadool J, et al. 1993. Dev. Dyn. 196:252.
3. Felzmann T, et al. 1991. J. Clin. Immunol. 11:205.
Gene ID: 682 View all products for this Gene ID
UniProt: View information about CD147 on UniProt.org

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