Ultra-LEAF™ Purified anti-human CD19 Antibod-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 3318	1

Product Details

Verified Reactivity

Human

Reported Reactivity

Chimpanzee

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/?g of the protein (< 0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
CyTOF? - Verified
Block, IHC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 ?g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections⁸ and blocking of B cell proliferation. Clone HIB19 is not recommended for formalin-fixed paraffin-embedded sections. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 302267 & 302268).

Clone HIB19 partially blocks anti-human CD19 clones 4G7 and SJ25C1 staining based on in-house testing

Application References (PubMed link indicates BioLegend citation)

Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York.
Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
Bradbury L, et al. 1993. J. Immunol. 151:2915.
Joseph A, et al. 2010. J. Virol. 84:6645. PubMed
Wang X, et al. 2010. Haematologica. 95:884. (FC) PubMed
Walker JD, et al. 2009. J. Immunol. 182:1548. (Block) PubMed
Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Hansen A, et al. 2002. Arthritis Rheum. 46:2160. (IHC)
Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)

RRID

AB_2832579 (BioLegend Cat. No. 302267) AB_2832580 (BioLegend Cat. No. 302268)

Antigen Details

Structure: Ig superfamily, type I transmembrane glycoprotein, 95 kD
Distribution: B lineage (except plasma cells), follicular dendritic cells
Function: B cell activation and differentiation
Ligand/Receptor: Forms complex with CD21 (CR2) and CD81 (TAPA-1), BCR coreceptor
Cell Type: B cells, Dendritic cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Tedder T, et al. 1994. Immunol. Today 15:437.
2. Bradbury L, et al. 1993. J. Immunol. 151:2915.
Gene ID: 930 View all products for this Gene ID
UniProt: View information about CD19 on UniProt.org

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