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首页 > 抗体蛋白 > 抗体
PE/Cyanine7 anti-human CD19 Antibody
产品名称：
PE/Cyanine7 anti-human CD19 Antibody
产品类别：
抗体
产品编号：
302215
产品应用：
302215
[价格]
规格 价格 库存
25tests ￥ 2002 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections8 and blocking of B cell proliferation. Clone HIB19 is not recommended for formalin-fixed paraffin-embedded sections. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 302267 & 302268).

?

Clone HIB19 partially blocks anti-human CD19 clones 4G7 and SJ25C1 staining based on in-house testing

Additional Product Notes

BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  3. Bradbury L, et al. 1993. J. Immunol. 151:2915.
  4. Joseph A, et al. 2010. J. Virol. 84:6645. PubMed
  5. Wang X, et al. 2010. Haematologica. 95:884. (FC) PubMed
  6. Walker JD, et al. 2009. J. Immunol. 182:1548. (Block) PubMed
  7. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  8. Hansen A, et al. 2002. Arthritis Rheum. 46:2160. (IHC)
  9. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
  10. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Watanabe A, et al. 2019. Cell. 177:1124. PubMed
  2. Wu J, et al. 2022. Nat Commun. 13:676. PubMed
  3. Xu Y, et al. 2022. Nat Commun. 13:980. PubMed
  4. SoRelle ED, et al. 2022. Front Immunol. 13:1001145. PubMed
  5. Wijesinghe A, et al. 2020. J Biomed Sci. 27:50:00. PubMed
  6. Ryzhov S, et al. 2019. J Am Heart Assoc. 8:e010874. PubMed
  7. Li H, et al. 2018. PLoS Genet. 14:e1007163. PubMed
  8. Hubbard N, et al. 2016. Blood. 127: 2513 - 2522. PubMed
  9. Li C, et al. 2022. J Exp Med. 219:. PubMed
  10. Hao Y, et al. 2018. Exp Ther Med. 15:1403. PubMed
  11. Browning D, et al. 2017. Gene Ther. 10.1038/gt.2016.88. PubMed
  12. Li Y, et al. 2021. Nat Commun. 12:87. PubMed
  13. Gajdasik DW, et al. 2020. Nat Commun. 2.834027778. PubMed
  14. Joosten S, et al. 2016. PLoS Pathog. 12: 1005687. PubMed
  15. Tardif V, et al. 2019. Nat Commun. 10:823. PubMed
  16. Cillo AR, et al. 2020. Immunity. 52(1):183-199.e9.. PubMed
  17. Amara K, et al. 2013. J Exp Med. 210:445. PubMed
  18. Pereira JL, et al. 2021. Transl Oncol. 14:101125. PubMed
  19. van Heerden D, et al. 2021. Int J Mol Sci. 22:. PubMed
  20. Perelman SS, et al. 2021. Nat Microbiol. 6:731. PubMed
  21. Masuda T, et al. 2022. iScience. 25:104781. PubMed
  22. Jackson-Jones LH, et al. 2020. Immunity. 52:700. PubMed
  23. Dugan HL, et al. 2021. Immunity. 54(6):1290-1303.e7. PubMed
  24. Celik H, et al. 2021. Cancer Discov. 11:3126. PubMed
  25. Martín Monreal MT, et al. 2021. Front Immunol. 12:716250. PubMed
  26. Izmirly AM, et al. 2022. PLoS Pathog. 18:e1009903. PubMed
  27. Kornauth C, et al. 2021. Haematologica. 106:2251. PubMed
  28. Schr?der M, et al. 2016. PLoS One. 11: 0157387. PubMed
  29. Magri G et al. 2017. Immunity. 47(1):118-134 . PubMed
  30. Eccles JD, et al. 2020. Cell Rep. 30:351. PubMed
  31. Pellin D, et al. 2019. Nat Commun. 10:2395. PubMed
  32. Szklarski M, et al. 2021. Front Immunol. 12:644548. PubMed
  33. Lu E, et al. 2019. Nature. 567:244. PubMed
  34. Schiroli G et al. 2019. Cell stem cell. 24(4):551-565 . PubMed
  35. Mercer F, et al. 2016. PLoS Negl Trop Dis. 10: 0004913. PubMed
  36. Menon M, et al. 2016. Immunity. 44:683-697. PubMed
  37. Zhang CR, et al. 2022. Blood Cancer Discov. 3:220. PubMed
  38. Roy Chowdhury R, et al. 2018. Nature. 560:644. PubMed
  39. Lindner S, et al. 2013. Cancer Res. 73:2468. PubMed
  40. Huynh L, et al. 2016. Sci Rep. 6:31959. PubMed
  41. Janssen JJE, et al. 2022. Am J Physiol Endocrinol Metab. 322:E141. PubMed
  42. Shuwa HA, et al. 2021. Med. 2(6):720-735.e4. PubMed
  43. Rappazzo CG, et al. 2022. Immunity. 55:1710. PubMed
  44. Fels JM, et al. 2021. Cell. 184(13):3486-3501.e21. PubMed
  45. Zou J, et al. 2022. J Clin Invest. 132:. PubMed
  46. Song S, et al. 2021. Front Immunol. 12:705140. PubMed
  47. Csomós K, et al. 2022. Nat Immunol. 23:1256. PubMed
  48. Crippa S, et al. 2019. J Clin Invest. 129:1566. PubMed
  49. Napolitano R, et al. 2020. Oncol Rep. 44:1561. PubMed
  50. Dinh HQ, et al. 2020. Immunity. 53(2):319-334.e6. PubMed
  51. Sakharkar M, et al. 2021. Sci Immunol. :6. PubMed
  52. Connors J, et al. 2022. Geroscience. :. PubMed
  53. Rossi E, et al. 2014. PLoS One. 9:98315. PubMed
  54. Li X, et al. 2021. Front Immunol. 12:779560. PubMed
RRID
AB_314245 (BioLegend Cat. No. 302215) AB_314246 (BioLegend Cat. No. 302216)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 95 kD
Distribution

B lineage (except plasma cells), follicular dendritic cells

Function
B cell activation and differentiation
Ligand/Receptor
Forms complex with CD21 (CR2) and CD81 (TAPA-1), BCR coreceptor
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Tedder T, et al. 1994. Immunol. Today 15:437.
2. Bradbury L, et al. 1993. J. Immunol. 151:2915.

Gene ID
930 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org

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