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PE/Cyanine5 anti-human CD19 Antibody
产品名称：
PE/Cyanine5 anti-human CD19 Antibody
产品类别：
抗体
产品编号：
302209
产品应用：
302209
[价格]
规格 价格 库存
25tests ￥ 1624 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections8 and blocking of B cell proliferation. Clone HIB19 is not recommended for formalin-fixed paraffin-embedded sections. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 302267 & 302268).

?

Clone HIB19 partially blocks anti-human CD19 clones 4G7 and SJ25C1 staining based on in-house testing

Additional Product Notes

BioLegend is in the process of converting the name PE/Cy5 to PE/Cyanine5. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine5 products. Please contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  3. Bradbury L, et al. 1993. J. Immunol. 151:2915.
  4. Joseph A, et al. 2010. J. Virol. 84:6645. PubMed
  5. Wang X, et al. 2010. Haematologica. 95:884. (FC) PubMed
  6. Walker JD, et al. 2009. J. Immunol. 182:1548. (Block) PubMed
  7. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  8. Hansen A, et al. 2002. Arthritis Rheum. 46:2160. (IHC)
  9. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
  10. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Labuhn M, et al. 2020. Cancer Cell. 36(2):123-138. PubMed
  2. Lagou V et al. 2018. Cell reports. 25(3):798-810 . PubMed
  3. Geuijen C, et al. 2021. Nat Commun. 12:4445. PubMed
  4. Wu VH, et al. 2020. JCI Insight. 5:00. PubMed
  5. Boisson B, et al. 2015. J Exp Med. 212:939. PubMed
  6. Fletcher H, et al. 2016. Nat Commun. 7: 11290. PubMed
  7. Nilsson A, et al. 2016. PLoS One. 11: 0158369. PubMed
  8. Ratliff ML, et al. 2019. J Autoimmun. 96:158. PubMed
  9. Pan YG, et al. 2021. Immunity. 54(6):1245-1256.e5. PubMed
  10. Demers K, et al. 2016. PLoS Pathog. 12: 1005805. PubMed
  11. Alsaleh G, et al. 2020. Elife. 9: . PubMed
  12. Di Genua C, et al. 2020. Cancer Cell. 37(5):690-704.e8.. PubMed
  13. Sibener LV et al. 2018. Cell. 174(3):672-687 . PubMed
  14. Yuan Z, et al. 2018. Emerg Microbes Infect. 7:59. PubMed
  15. Cassetta L et al. 2019. Cancer Cell. 35(4):588-602 . PubMed
  16. Grifoni A, et al. 2020. Cell. 181(7):1489-1501.e15.. PubMed
  17. van den Hoogen P, et al. 2020. PLoS One. 14:e0227283. PubMed
  18. Ziegler CGK, et al. 2021. Cell. 184(18):4713-4733.e22. PubMed
  19. Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed
  20. Wang L, et al. 2014. Proc Natl Acad Sci U S A. 111:3146. PubMed
  21. Franco LM, et al. 2019. J Exp Med. 216:384. PubMed
  22. Jung J, et al. 2019. Cell Rep. 26:1906. PubMed
  23. Rydyznski Moderbacher C, et al. 2020. Cell. 183(4):996-1012.e19. PubMed
  24. Yuan Z, et al. 2016. J Virol. 90: 7728 - 7739. PubMed
  25. Bill M, et al. 2018. J Cell Mol Med. 22:2311. PubMed
  26. Gupta R, et al. 2020. Cell Stem Cell. 26:527. PubMed
  27. Rodriguez‐Meira A et al. 2019. Molecular cell. 73(6):1292-1305 . PubMed
RRID
AB_314239 (BioLegend Cat. No. 302209) AB_314240 (BioLegend Cat. No. 302210)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 95 kD
Distribution

B lineage (except plasma cells), follicular dendritic cells

Function
B cell activation and differentiation
Ligand/Receptor
Forms complex with CD21 (CR2) and CD81 (TAPA-1), BCR coreceptor
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Tedder T, et al. 1994. Immunol. Today 15:437.
2. Bradbury L, et al. 1993. J. Immunol. 151:2915.

Gene ID
930 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org

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