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Pacific Blue™ anti-human CD19 Antibody
产品名称：
Pacific Blue™ anti-human CD19 Antibody
产品类别：
抗体
产品编号：
302223
产品应用：
302223
[价格]
规格 价格 库存
25µg ￥ 1736 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
test size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
?g sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue? under optimal conditions.
Concentration
test size: lot-specific; ?g sizes: 0.5 mg/ml
Storage & Handling
The CD19 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis.
For test sizes, the suggested use of this reagent for immunofluorescent staining is 5 ?l per 106 cells in 100 ?l volume.
For ?g sizes, the suggested use of this reagent for immunofluorescent staining is ≤ 0.5 ?g per 106 cells in 100 ?l volume.
It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections8 and blocking of B cell proliferation. Clone HIB19 is not recommended for formalin-fixed paraffin-embedded sections. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 302267 & 302268).

?

Clone HIB19 partially blocks anti-human CD19 clones 4G7 and SJ25C1 staining based on in-house testing

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  3. Bradbury L, et al. 1993. J. Immunol. 151:2915.
  4. Joseph A, et al. 2010. J. Virol. 84:6645. PubMed
  5. Wang X, et al. 2010. Haematologica. 95:884. (FC) PubMed
  6. Walker JD, et al. 2009. J. Immunol. 182:1548. (Block) PubMed
  7. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  8. Hansen A, et al. 2002. Arthritis Rheum. 46:2160. (IHC)
  9. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
  10. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Fu J et al. 2019. Cell stem cell. 24(2):227-239 . PubMed
  2. Alcántara‐Hernández M et al. 2017. Immunity. 47(6):1037-1050 . PubMed
  3. Savage TM, et al. 2018. JCI Insight. 3:. PubMed
  4. Macal M, et al. 2016. J Immunol. 196: 1900 - 1909. PubMed
  5. Boer M, et al. 2015. Clin Vaccine Immunol. 22: 778 - 788. PubMed
  6. Greenshields-Watson A, et al. 2020. Cell Rep. 32:107885. PubMed
  7. Chen YQ et al. 2018. Cell. 173(2):417-429 . PubMed
  8. Dockree T, et al. 2016. Immunol Cell Biol. 95:68-76. PubMed
  9. Yang Z, et al. 2018. Front Immunol. 9:2613. PubMed
  10. Queckborner S, et al. 2020. Stem Cell Res Ther. 11:15. PubMed
  11. Koning D, et al. 2013. J Immunol. 190:391. PubMed
  12. Gauvin J, et al. 2016. PLoS One. 11: 0155868. PubMed
  13. Dolton G, et al. 2022. Cell. 185:2936. PubMed
  14. Ogishi M, et al. 2021. Nat Med. 27:1646. PubMed
  15. Lu Y, et al. 2020. Cell. 180(6):1081-1097. PubMed
  16. Chen L, et al. 2016. Ann Rheum Dis. 75: 916 - 923. PubMed
  17. Vetsika EK, et al. 2021. Cancers (Basel). 13:. PubMed
  18. Santos J, et al. 2013. J Transl Med. 11:18. PubMed
  19. Osorio FG et al. 2018. Cell reports. 25(9):2308-2316 . PubMed
  20. Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed
  21. Della-Torre E, et al. 2015. Ann Rheum Dis. 74: 2236 - 2243. PubMed
  22. Ying Zhang et al. 2017. Cancer cell. 32(3):377-391 . PubMed
  23. Tungatt K, et al. 2015. J Immunol. 194:463. PubMed
  24. Zuccato C, et al. 2022. Ther Adv Hematol. 13:20406207221100648. PubMed
  25. Autenrieth S, et al. 2015. Clin Transl Immunology. 4:50. PubMed
  26. Barman S, et al. 2016. Int Immunol. 28: 533 - 545. PubMed
  27. Lee-Chang C, et al. 2021. J Exp Med. 218:. PubMed
  28. Rouers A, et al. 2021. iScience. 24:102482. PubMed
  29. Linley A, et al. 2015. Blood. 126: 1902 - 1910. PubMed
  30. Dulberger CL et al. 2017. Immunity. 46(6):1018-1029 . PubMed
  31. Rius C, et al. 2018. J Immunol. 200:2263. PubMed
  32. Darcis G, et al. 2020. Cell Rep. 30:2284. PubMed
  33. Bartholomaeus P, et al. 2014. J Immunol. 192:2091. PubMed
  34. Drennan S, et al. 2016. Leukemia. 10.1038/leu.2016.356. PubMed
  35. Holland C, et al. 2015. J Immunol. 195: 5827 - 5836. PubMed
  36. Steinsb? &, et al. 2014. Nat Commun. 5:4041. PubMed
  37. Yang C, et al. 2019. Nat Commun. 10:3931. PubMed
  38. Serr I, et al. 2016. Nat Commun. 7:10991. PubMed
  39. Li M, et al. 2020. Nat Commun. 4051:11. PubMed
  40. Lindeman I, et al. 2021. J Exp Med. 218:. PubMed
  41. Longino NV, et al. 2019. Cancer Immunol Res. 7:1727. PubMed
  42. Welch JL, et al. 2020. FEBS Lett. 594:695. PubMed
  43. Malarkey C, et al. 2016. PLoS One. 11: 0157157. PubMed
  44. Shao S, et al. 2020. Proc Natl Acad Sci U S A. 117:8845. PubMed
RRID
AB_493652 (BioLegend Cat. No. 302223) AB_2073118 (BioLegend Cat. No. 302232) AB_493653 (BioLegend Cat. No. 302224)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 95 kD
Distribution

B lineage (except plasma cells), follicular dendritic cells

Function
B cell activation and differentiation
Ligand/Receptor
Forms complex with CD21 (CR2) and CD81 (TAPA-1), BCR coreceptor
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Tedder T, et al. 1994. Immunol. Today 15:437.
2. Bradbury L, et al. 1993. J. Immunol. 151:2915.

Gene ID
930 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org

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