Biotin anti-human CD19 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 504	1

Product Details

Verified Reactivity

Human

Reported Reactivity

Chimpanzee

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections⁸ and blocking of B cell proliferation. Clone HIB19 is not recommended for formalin-fixed paraffin-embedded sections. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 302267 & 302268).

Clone HIB19 partially blocks anti-human CD19 clones 4G7 and SJ25C1 staining based on in-house testing

Application References (PubMed link indicates BioLegend citation)

Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York.
Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
Bradbury L, et al. 1993. J. Immunol. 151:2915.
Joseph A, et al. 2010. J. Virol. 84:6645. PubMed
Wang X, et al. 2010. Haematologica. 95:884. (FC) PubMed
Walker JD, et al. 2009. J. Immunol. 182:1548. (Block) PubMed
Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Hansen A, et al. 2002. Arthritis Rheum. 46:2160. (IHC)
Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)

Product Citations

Garcia–Perez JE, et al. 2019. Front Immunol. 10:998. PubMed
Riether C, et al. 2021. Cell Reports. 34(4):108663. PubMed
Kerr SC, et al. 2020. Clin Exp Allergy. 50:904. PubMed
Hinterbrandner M, et al. 2021. JCI Insight. 6:e151797. PubMed
Evren E, et al. 2020. Immunity. 54(2):259-275.e7. PubMed
Joosten S, et al. 2016. PLoS Pathog. 12: 1005687. PubMed
Bourdely P, et al. 2020. Immunity. 53(2):335-352. PubMed
Xhangolli I, et al. 2019. Genomics Proteomics Bioinformatics. 17:129. PubMed
Yankova E, et al. 2021. Nature. 593:597. PubMed
Omer OS, et al. 2020. Methods Mol Biol. 2121:199. PubMed
Liu Y, et al. 2022. Front Oncol. 12:1037934. PubMed
Ye CJ, et al. 2018. Genome Res. 28:1812. PubMed
Hirota K et al. 2018. Immunity. 48(6):1220-1232 . PubMed
Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
Zhang B, et al. 2021. Nature. 599:471. PubMed

RRID

AB_314233 (BioLegend Cat. No. 302203) AB_314234 (BioLegend Cat. No. 302204)

Antigen Details

Structure: Ig superfamily, type I transmembrane glycoprotein, 95 kD
Distribution: B lineage (except plasma cells), follicular dendritic cells
Function: B cell activation and differentiation
Ligand/Receptor: Forms complex with CD21 (CR2) and CD81 (TAPA-1), BCR coreceptor
Cell Type: B cells, Dendritic cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Tedder T, et al. 1994. Immunol. Today 15:437.
2. Bradbury L, et al. 1993. J. Immunol. 151:2915.
Gene ID: 930 View all products for this Gene ID
UniProt: View information about CD19 on UniProt.org

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