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Alexa Fluor® 700 anti-human CD19 Antibody
产品名称：
Alexa Fluor® 700 anti-human CD19 Antibody
产品类别：
抗体
产品编号：
302225
产品应用：
302225
[价格]
规格 价格 库存
25µg ￥ 1764 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor? 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections8 and blocking of B cell proliferation. Clone HIB19 is not recommended for formalin-fixed paraffin-embedded sections. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 302267 & 302268).

?

Clone HIB19 partially blocks anti-human CD19 clones 4G7 and SJ25C1 staining based on in-house testing

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  3. Bradbury L, et al. 1993. J. Immunol. 151:2915.
  4. Joseph A, et al. 2010. J. Virol. 84:6645. PubMed
  5. Wang X, et al. 2010. Haematologica. 95:884. (FC) PubMed
  6. Walker JD, et al. 2009. J. Immunol. 182:1548. (Block) PubMed
  7. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  8. Hansen A, et al. 2002. Arthritis Rheum. 46:2160. (IHC)
  9. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
  10. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
  2. Ivan Jelcic et al. 2018. Cell. 175(1):85-100 . PubMed
  3. Fasbender F, et al. 2017. Front Immunol. 0.88125. PubMed
  4. Brouwer PJM, et al. 2020. Science. 369:643. PubMed
  5. Schmitz T, et al. 2021. J Reprod Immunol. 148:103424. PubMed
  6. Quast I, et al. 2015. Neurol Neuroimmunol Neuroinflamm. 2: e148. PubMed
  7. Wang J, et al. 2020. Cell. 183:1264. PubMed
  8. Jagannathan P, et al. 2017. Sci Rep. 10.1038/s41598-017-10624-3. PubMed
  9. Tardif V, et al. 2019. Nat Commun. 10:823. PubMed
  10. Boyle M, et al. 2015. J Infect Dis. 212: 416-425. PubMed
  11. Miles B, et al. 2015. Nat Commun. 6: 8608. PubMed
  12. Rubio-Pérez C, et al. 2021. Nat Commun. 1503:12. PubMed
  13. Feldman J, et al. 2021. Sci Immunol. 6:eabl5842. PubMed
  14. Shin JJ, et al. 2022. J Clin Immunol. :. PubMed
  15. Caduff N, et al. 2021. Cell Reports. 35(5):109056. PubMed
  16. Ramos RN, et al. 2022. Clin Transl Immunology. 11:e1392. PubMed
  17. Urlaub D, et al. 2019. Arthritis Res Ther. 1.067361111. PubMed
  18. Sefik E, et al. 2021. Nat Biotechnol. . PubMed
  19. Port JR, et al. 2020. J Virol. 94:. PubMed
  20. Gorman JA, et al. 2019. Front Immunol. 10:44. PubMed
  21. Berry MR et al. 2017. Cell. 170(5):860-874 . PubMed
  22. Shin CH, et al. 2020. J Immunol. 205:3179. PubMed
  23. Hood SP, et al. 2020. Elife. 9:00. PubMed
  24. Teng F, et al. 2021. Cell Rep. 37:110051. PubMed
  25. Plum T, et al. 2020. Immunity. 52(2):404-416. PubMed
  26. Inglis H, et al. 2015. J Vis Exp. 97: 52484. PubMed
  27. Serr I, et al. 2016. Nat Commun. 7:10991. PubMed
  28. Harris LD, et al. 2020. Front Cell Infect Microbiol. 10:120. PubMed
  29. Faissner S, et al. 2022. Front Immunol. 13:980526. PubMed
RRID
AB_493750 (BioLegend Cat. No. 302225) AB_493751 (BioLegend Cat. No. 302226)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 95 kD
Distribution

B lineage (except plasma cells), follicular dendritic cells

Function
B cell activation and differentiation
Ligand/Receptor
Forms complex with CD21 (CR2) and CD81 (TAPA-1), BCR coreceptor
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Tedder T, et al. 1994. Immunol. Today 15:437.
2. Bradbury L, et al. 1993. J. Immunol. 151:2915.

Gene ID
930 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org

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