Purified anti-human CD95 (Fas) Antibody
- 产品名称：
- Purified anti-human CD95 (Fas) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 305602
- 产品应用：
- 305602
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- African Green, Baboon, Capuchin Monkey, Chimpanzee, Common Marmoset, Cotton-topped Tamarin, Pigtailed Macaque, Sooty Mangabey
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- CD95 transfected L cells
- Formulation
- Phosphate-buffered solution, pH7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
ICC?- Verified
IHC - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per million cells in 100 ?L?volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
The DX2 antibody is useful for inducing apoptosis of Fas-positive cells. Additional reported applications (for the relevant formats) include: in vitro induction of apoptosis3 (DX2 antibody is required to be cross-linked for effective induction of apoptosis) and immunohistochemical staining4,5 of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 305655 and 305656).
Note: EOS9.1 antibody can induce apoptosis without cross-linking.
- Application References
(PubMed link indicates BioLegend citation) -
- Schlossman S, et al. Eds.1995. Leucocyte Typing V. Oxford University Press. New York.
- Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. New York.
- Cifone M, et al. 1994. J. Exp. Med. 180:1547. (Apop)
- Zietz C, et al. 2001. Am. J. Pathol. 159:963. (IHC)
- Sergi C, et al. 2000. Am. J. Pathol. 156:1589. (IHC)
- Xie S, et al. 2010. J. Immunol. 184:2289. (FC) PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21.
- Rout N, et al. 2010. PLoS One 5:e9787. (FC)
- Dixit N, et al. 2012. J. Immunol. 189:5954. PubMed
- Product Citations
-
- Dixit N, et al. 2012. J Immunol. 189:5954. PubMed
- Ortiz AM, et al. 2018. Nat Med. 24:1313. PubMed
- Sharma S, et al. 2019. Nat Commun. 2.572916667. PubMed
- Nugent JL, et al. 2021. iScience. 24:103421. PubMed
- Kaufmann M, et al. 2021. Med. 2(3):296-312.e8. PubMed
- Ortiz AM, et al. 2022. Sci Rep. 12:7491. PubMed
- Zhang Y, et al. 2020. Front Immunol. 11:1012. PubMed
- Syrimi E, et al. 2021. iScience. 24:103215. PubMed
- O'Boyle KC, et al. 2020. Methods Mol Biol. 2111:1. PubMed
- Sun Y, et al. 2020. J Immunol. 205:3358. PubMed
- Eccles JD, et al. 2020. Cell Rep. 30:351. PubMed
- Chng MHY, et al. 2020. Immunity. 51(6):1119-1135.e5.. PubMed
- Sciuto MR, et al. 2018. Mol Cell Proteomics. 1.397916667. PubMed
- De Maeyer RPH, et al. 2020. Nat Immunol. 21:615. PubMed
- RRID
- AB_314540 (BioLegend Cat. No. 305602)
- Structure
- TNFR superfamily, type I transmembrane protein, 45 kD
- Distribution
-
T cells and B cells, monocytes, neutrophils, fibroblasts, expression level upregulated on activated lymphocytes
- Function
- Mediates apoptosis
- Ligand/Receptor
- Fas ligand (CD178)
- Cell Type
- B cells, Fibroblasts, Lymphocytes, Monocytes, Neutrophils, T cells
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroscience
- Molecular Family
- CD Molecules
- Antigen References
-
1. Krammer P, et al. 1994. Immunol. Rev. 142:175.
2. Nagata S, et al. 1995. Science 267:1449.
- Gene ID
- 355 View all products for this Gene ID
- UniProt
- View information about CD95 on UniProt.org
