PE/Fire™ 640 anti-human CD95 (Fas) Antibody
- 产品名称：
- PE/Fire™ 640 anti-human CD95 (Fas) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 305657
- 产品应用：
- 305657
[价格]
| 规格 |
价格 |
库存 |
| 25tests |
￥ 2058 |
1 |
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- African Green, Baboon, Capuchin Monkey, Chimpanzee, Common Marmoset, Cotton-topped Tamarin, Pigtailed Macaque, Sooty Mangabey
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- CD95 transfected L cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Fire? 640 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?L per million cells in 100 ?L staining volume or 5 ?L per 100 ?L of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* PE/Fire? 640 has a maximum excitation of 566 nm and a maximum emission of 639 nm.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
The DX2 antibody is useful for inducing apoptosis of Fas-positive cells. Additional reported applications (for the relevant formats) include: in vitro induction of apoptosis3 (DX2 antibody is required to be cross-linked for effective induction of apoptosis) and immunohistochemical staining4,5 of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 305655 and 305656).
Note: EOS9.1 antibody can induce apoptosis without cross-linking.
- Application References
(PubMed link indicates BioLegend citation) -
- Schlossman S, et al. Eds.1995. Leucocyte Typing V. Oxford University Press. New York.
- Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. New York.
- Cifone M, et al. 1994. J. Exp. Med. 180:1547. (Apop)
- Zietz C, et al. 2001. Am. J. Pathol. 159:963. (IHC)
- Sergi C, et al. 2000. Am. J. Pathol. 156:1589. (IHC)
- Xie S, et al. 2010. J. Immunol. 184:2289. (FC) PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21.
- Rout N, et al. 2010. PLoS One 5:e9787. (FC)
- Dixit N, et al. 2012. J. Immunol. 189:5954. PubMed
- RRID
- AB_2892365 (BioLegend Cat. No. 305657) AB_2892365 (BioLegend Cat. No. 305658)
- Structure
- TNFR superfamily, type I transmembrane protein, 45 kD
- Distribution
-
T cells and B cells, monocytes, neutrophils, fibroblasts, expression level upregulated on activated lymphocytes
- Function
- Mediates apoptosis
- Ligand/Receptor
- Fas ligand (CD178)
- Cell Type
- B cells, Fibroblasts, Lymphocytes, Monocytes, Neutrophils, T cells
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroscience
- Molecular Family
- CD Molecules
- Antigen References
-
1. Krammer P, et al. 1994. Immunol. Rev. 142:175.
2. Nagata S, et al. 1995. Science 267:1449.
- Gene ID
- 355 View all products for this Gene ID
- UniProt
- View information about CD95 on UniProt.org
