PE anti-human CD95 (Fas) Antibody
- 产品名称：
- PE anti-human CD95 (Fas) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 305607
- 产品应用：
- 305607
[价格]
| 规格 |
价格 |
库存 |
| 25tests |
￥ 1806 |
1 |
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- African Green, Baboon, Capuchin Monkey, Chimpanzee, Common Marmoset, Cotton-topped Tamarin, Pigtailed Macaque, Sooty Mangabey
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- CD95 transfected L cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
The DX2 antibody is useful for inducing apoptosis of Fas-positive cells. Additional reported applications (for the relevant formats) include: in vitro induction of apoptosis3 (DX2 antibody is required to be cross-linked for effective induction of apoptosis) and immunohistochemical staining4,5 of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 305655 and 305656).
Note: EOS9.1 antibody can induce apoptosis without cross-linking.
- Application References
(PubMed link indicates BioLegend citation) -
- Schlossman S, et al. Eds.1995. Leucocyte Typing V. Oxford University Press. New York.
- Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. New York.
- Cifone M, et al. 1994. J. Exp. Med. 180:1547. (Apop)
- Zietz C, et al. 2001. Am. J. Pathol. 159:963. (IHC)
- Sergi C, et al. 2000. Am. J. Pathol. 156:1589. (IHC)
- Xie S, et al. 2010. J. Immunol. 184:2289. (FC) PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21.
- Rout N, et al. 2010. PLoS One 5:e9787. (FC)
- Dixit N, et al. 2012. J. Immunol. 189:5954. PubMed
- Product Citations
-
- Li H, et al. 2016. J Immunol. 196: 4064 - 4074. PubMed
- Policicchio B, et al. 2016. PLoS Pathog. 12: 1005879. PubMed
- Radhakrishnan H, et al. 2017. Cancer Lett. 10.1016/j.canlet.2017.06.020. PubMed
- Ng W, et al. 2021. Pharm Biol. 59:47:00. PubMed
- Hu W, et al. 2012. Asian J Androl. 14:695. PubMed
- Foight GW, et al. 2019. Nat Biotechnol. 37:1209. PubMed
- Wang LW, et al. 2018. Curr Protoc Mol Biol. 121:31.13.1. PubMed
- Li N, et al. 2021. Cell Rep Med. 2:100297. PubMed
- Xie S, et al. 2010. J Immunol. 184:2289. PubMed
- Flint S, et al. 2016. Haematologica. 101: 698 - 706. PubMed
- Yao C, et al. 2018. Autophagy. 1.854861111. PubMed
- Lehner M, et al. 2007. Leuk Res. 31:1729. PubMed
- Szodoray P, et al. 2021. Cell Reports. 36(6):109525. PubMed
- Wang X, et al. 2022. J Exp Clin Cancer Res. 41:210. PubMed
- Alhaj Hussen K, et al. 2020. Front Immunol. 11:579776. PubMed
- Kasatskaya SA, et al. 2020. Elife. :9. PubMed
- RRID
- AB_314545 (BioLegend Cat. No. 305607) AB_314546 (BioLegend Cat. No. 305608)
- Structure
- TNFR superfamily, type I transmembrane protein, 45 kD
- Distribution
-
T cells and B cells, monocytes, neutrophils, fibroblasts, expression level upregulated on activated lymphocytes
- Function
- Mediates apoptosis
- Ligand/Receptor
- Fas ligand (CD178)
- Cell Type
- B cells, Fibroblasts, Lymphocytes, Monocytes, Neutrophils, T cells
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroscience
- Molecular Family
- CD Molecules
- Antigen References
-
1. Krammer P, et al. 1994. Immunol. Rev. 142:175.
2. Nagata S, et al. 1995. Science 267:1449.
- Gene ID
- 355 View all products for this Gene ID
- UniProt
- View information about CD95 on UniProt.org
