Purified anti-PINK1 Antibody
- 产品名称：
- Purified anti-PINK1 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 846201
- 产品应用：
- 846201
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 5.0 - 10 ?g per mL.?For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Product Citations
-
- Bharath LP, et al. 2020. Cell Metabolism. 32(1):44-55.e6.. PubMed
- Chung E, et al. 2020. Sci Adv. 6:eaba1193. PubMed
- RRID
- AB_2783414 (BioLegend Cat. No. 846201) AB_2572127 (BioLegend Cat. No. 846202)
- Structure
- PINK1 is a 581 amino acid, 63 kD, transmembrane protein that contains an N-terminal mitochondrial localization sequence, a serine/threonine kinase domain, and a C-terminal regulatory domain. PINK1 is proteolytically cleaved by PARL into a 55 kD fragment.
- Distribution
-
Mitochondria and cytoplasm.
- Function
- PINK1 is involved in the clearance of damaged mitochondria via selective autophagy (mitophagy) by mediating activation and translocation of Parkin protein (PARK2). PARL-cleaved PINK1 is a cytoplasmic probe for damaged mitochondria. PINK1 phosphorylation of mitochondrial proteins can protect against mitochondrial dysfunction due to cellular stresses.
- Interaction
- PARKIN, Beclin 1, FBX07, DJ-1, HSP90/CDC37.
- Ligand/Receptor
- PARKIN, Beclin 1, FBX07, DJ-1, HSP90/CDC37.
- Biology Area
- Cell Biology, Mitochondrial Function, Neurodegeneration, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Protein Trafficking and Clearance
- Molecular Family
- Mitochondrial Markers
- Antigen References
-
1. Trempe JF, Fon EA. 2013. Front Neurol. 4:38.
2. Durcan TM, Fon EA. 2015. Genes Dev. 29(10):989.
- Gene ID
- 65018 View all products for this Gene ID
- UniProt
- View information about PINK1 on UniProt.org
