Ultra-LEAF™ Purified anti-mouse CD357 (GITR)
- 产品名称：
- Ultra-LEAF™ Purified anti-mouse CD357 (GITR)
- 产品类别：
- 抗体
- 产品编号：
- 126321
- 产品应用：
- 126321
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse CD25+ CD4+ T cells
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
IP, FA - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application References
(PubMed link indicates BioLegend citation) -
- De Luca A, et al. 2007. J. Immunol. 179:5999. PubMed
- RRID
- AB_2814005 (BioLegend Cat. No. 126321) AB_2814006 (BioLegend Cat. No. 126322) AB_2814007 (BioLegend Cat. No. 126323) AB_2814008 (BioLegend Cat. No. 126324) AB_2814009 (BioLegend Cat. No. 126325) AB_2814010 (BioLegend Cat. No. 126326)
- Structure
- 66-70 kD homodimer of TNFR superfamily member.
- Distribution
-
Low levels on resting T cells, B cells, and macrophages; high levels on CD25+ CD4+ Tregs and CD25+ CD4+ CD8- thymocytes.
- Function
- Stimulation of GITR can abrogate or reduce the inhibitory function of CD25+ CD4+ Treg cells. In vivo activation of GITR may lead to the development of autoimmune diseases.
- Ligand/Receptor
- GITR ligand (GITRL).
- Cell Type
- B cells, Macrophages, T cells, Thymocytes, Tregs
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Tone M, et al. 2003. Proc. Natl. Acad. Sci. USA 100:15059.
2. Shimizu J, et al. 2002 Nat. Immunol. 3:135.
3. Stephens GL, et al. 2004. J. Immunol. 173:5008.
4. McHugh RS, et al. 2002. Immunity 16:311.
- Regulation
- Activation of T and B cells can upregulate GITR expression.
- Gene ID
- 21936 View all products for this Gene ID
- UniProt
- View information about CD357 on UniProt.org
