PE anti-mouse CD357 (GITR) Antibody
- 产品名称：
- PE anti-mouse CD357 (GITR) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 126309
- 产品应用：
- 126309
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse CD25+ CD4+ T cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application References
(PubMed link indicates BioLegend citation) -
- De Luca A, et al. 2007. J. Immunol. 179:5999. PubMed
- Product Citations
-
- Wu J et al. 2017. Immunity. 47(6):1114-1128 . PubMed
- Lacal P, et al. 2013. J Pharmacol Exp Ther. 347:164. PubMed
- Schmidleithner L et al. 2019. Immunity. 50(5):1232-1248 . PubMed
- Palazon A, et al. 2017. Cancer Cell. . 10.1016/j.ccell.2017.10.003. PubMed
- Di Lorenzo A, et al. 2022. Oncoimmunology. 11:2086752. PubMed
- Wagner AK, et al. 2022. iScience. 25:105137. PubMed
- Timilshina M, et al. 2020. Cell Reports. 27(10):2948-2961.e7.. PubMed
- RRID
- AB_1089132 (BioLegend Cat. No. 126309) AB_1089130 (BioLegend Cat. No. 126310)
- Structure
- 66-70 kD homodimer of TNFR superfamily member.
- Distribution
-
Low levels on resting T cells, B cells, and macrophages; high levels on CD25+ CD4+ Tregs and CD25+ CD4+ CD8- thymocytes.
- Function
- Stimulation of GITR can abrogate or reduce the inhibitory function of CD25+ CD4+ Treg cells. In vivo activation of GITR may lead to the development of autoimmune diseases.
- Ligand/Receptor
- GITR ligand (GITRL).
- Cell Type
- B cells, Macrophages, T cells, Thymocytes, Tregs
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Tone M, et al. 2003. Proc. Natl. Acad. Sci. USA 100:15059.
2. Shimizu J, et al. 2002 Nat. Immunol. 3:135.
3. Stephens GL, et al. 2004. J. Immunol. 173:5008.
4. McHugh RS, et al. 2002. Immunity 16:311.
- Regulation
- Activation of T and B cells can upregulate GITR expression.
- Gene ID
- 21936 View all products for this Gene ID
- UniProt
- View information about CD357 on UniProt.org
