APC anti-mouse CD357 (GITR) Antibody
- 产品名称：
- APC anti-mouse CD357 (GITR) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 126311
- 产品应用：
- 126311
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse CD25+ CD4+ T cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Red Laser (633 nm)
- Application References
(PubMed link indicates BioLegend citation) -
- De Luca A, et al. 2007. J. Immunol. 179:5999. PubMed
- Product Citations
-
- Jayachandran R, et al. 2019. Immunity. 50:152. PubMed
- Alissafi T, et al. 2018. J Clin Invest. 128:3840. PubMed
- Alissafi T, et al. 2020. Cell Metabolism. 32(4):591-604.e7. PubMed
- Sasaki K, et al. 2019. Nat Commun. 10:3878. PubMed
- Cheng B, et al. 2022. Cancer Commun (Lond). 42:17. PubMed
- Knizkova D, et al. 2022. Nat Immunol. 23:1644. PubMed
- Katagiri T, et al. 2019. Mucosal Immunol. 12:p1104. PubMed
- Fan MY et al. 2018. Cell reports. 25(5):1204-1213 . PubMed
- RRID
- AB_1134212 (BioLegend Cat. No. 126311) AB_2271858 (BioLegend Cat. No. 126312)
- Structure
- 66-70 kD homodimer of TNFR superfamily member.
- Distribution
-
Low levels on resting T cells, B cells, and macrophages; high levels on CD25+ CD4+ Tregs and CD25+ CD4+ CD8- thymocytes.
- Function
- Stimulation of GITR can abrogate or reduce the inhibitory function of CD25+ CD4+ Treg cells. In vivo activation of GITR may lead to the development of autoimmune diseases.
- Ligand/Receptor
- GITR ligand (GITRL).
- Cell Type
- B cells, Macrophages, T cells, Thymocytes, Tregs
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Tone M, et al. 2003. Proc. Natl. Acad. Sci. USA 100:15059.
2. Shimizu J, et al. 2002 Nat. Immunol. 3:135.
3. Stephens GL, et al. 2004. J. Immunol. 173:5008.
4. McHugh RS, et al. 2002. Immunity 16:311.
- Regulation
- Activation of T and B cells can upregulate GITR expression.
- Gene ID
- 21936 View all products for this Gene ID
- UniProt
- View information about CD357 on UniProt.org
