PE/Cyanine7 anti-human CD62L Antibody
- 产品名称：
- PE/Cyanine7 anti-human CD62L Antibody
- 产品类别：
- 抗体
- 产品编号：
- 304821
- 产品应用：
- 304821
[价格]
| 规格 |
价格 |
库存 |
| 25tests |
￥ 2142 |
1 |
- Verified Reactivity
- Human
- Reported Reactivity
- Chimpanzee, Cow
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Concentrated supernatant from PMA-activated human peripheral blood leukocytes
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: Western blotting2,3,9 and in vitro blocking of lymphocytes binding to high endothelial venules (HEV)2. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 304853-304858).
- Additional Product Notes
- BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Kishimoto TK, et al. 1990. Proc. Natl. Acad. Sci. USA 87:2244. (WB, Block)
- Jutila M, et al. 2002. J. Immunol. 169:1768. (WB)
- Tamassia N, et al. 2008. J. Immunol. 181:6563. (FC) PubMed
- Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
- Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
- Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Koenig JM, et al. 1996. Pediatr. Res. 39:616. (WB)
- Shi C, et al. 2011. J. Immunol. 187:5293. (FC) PubMed
- Burges M, et al. 2013. Clin Cancer Res. 19:5675. PubMed
- Cash JL, et al. 2013. EMBO Rep. 14:999. (FC) PubMed
- Product Citations
-
- Chabi S, et al. 2020. Cell Reports. 29(8):2307-2320.e6.. PubMed
- Das RK, et al. 2019. Cancer Discov. 9:492. PubMed
- Kim C, et al. 2018. Cell Rep. 25:2148. PubMed
- Konadu K, et al. 2015. J Infect Dis. 211:1712. PubMed
- Shi Z, et al. 2021. Ann Clin Transl Neurol. 8:43. PubMed
- Giamarellos-Bourboulis EJ, et al. 2020. Cell. 183(2):315-323.e9. PubMed
- Toews K, et al. 2020. Mol Carcinog. 724:59. PubMed
- Moorlag SJCFM, et al. 2020. Cell Rep. 33:108387. PubMed
- Zhu Y, et al. 2019. Cell Stem Cell. 25:542. PubMed
- Li YR, et al. 2021. Cell Rep Med. 2:100449. PubMed
- Hu N, et al. 2014. PLoS One. 9:99671. PubMed
- Singh N, et al. 2016. Sci Transl Med. 8: 320ra3. PubMed
- Veerapathran A, et al. 2011. Blood. 118:5671. PubMed
- Liao HQ, et al. 2021. Am J Reprod Immunol. 86:e13406. PubMed
- Crompton J, et al. 2015. Cancer Res. 75:296. PubMed
- Zou F, et al. 2019. Nat Commun. 10:4109. PubMed
- RRID
- AB_830800 (BioLegend Cat. No. 304821) AB_830801 (BioLegend Cat. No. 304822)
- Structure
- Selectin, single chain glycoprotein, 74-95 kD
- Distribution
-
Majority of B cells, na?ve T cells, subset of memory T and NK cells, monocytes, granulocytes, thymocytes
- Function
- Leukocyte homing, leukocyte tethering, rolling
- Ligand/Receptor
- CD34, GlyCAM, MAdCAM-1
- Cell Type
- B cells, Granulocytes, Monocytes, Neutrophils, NK cells, T cells, Thymocytes, Tregs
- Biology Area
- Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
- Kishimoto T, et al. 1990. P. Natl. Acad. Sci. USA 87:2244.
- Kishimoto T, et al. 1991. Blood 78:805.
?
- Gene ID
- 6402 View all products for this Gene ID
- UniProt
- View information about CD62L on UniProt.org
