APC/Cyanine7 anti-human CD62L Antibody
- 产品名称：
- APC/Cyanine7 anti-human CD62L Antibody
- 产品类别：
- 抗体
- 产品编号：
- 304813
- 产品应用：
- 304813
[价格]
| 规格 |
价格 |
库存 |
| 25tests |
￥ 2142 |
1 |
- Verified Reactivity
- Human
- Reported Reactivity
- Chimpanzee, Cow
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Concentrated supernatant from PMA-activated human peripheral blood leukocytes
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
- Excitation Laser
- Red Laser (633 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: Western blotting2,3,9 and in vitro blocking of lymphocytes binding to high endothelial venules (HEV)2. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 304853-304858).
- Additional Product Notes
- BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Kishimoto TK, et al. 1990. Proc. Natl. Acad. Sci. USA 87:2244. (WB, Block)
- Jutila M, et al. 2002. J. Immunol. 169:1768. (WB)
- Tamassia N, et al. 2008. J. Immunol. 181:6563. (FC) PubMed
- Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
- Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
- Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Koenig JM, et al. 1996. Pediatr. Res. 39:616. (WB)
- Shi C, et al. 2011. J. Immunol. 187:5293. (FC) PubMed
- Burges M, et al. 2013. Clin Cancer Res. 19:5675. PubMed
- Cash JL, et al. 2013. EMBO Rep. 14:999. (FC) PubMed
- Product Citations
-
- Ajith A, et al. 2021. Front Immunol. 12:687715. PubMed
- Carre C, et al. 2021. iScience. 24:102970. PubMed
- Ruck T, et al. 2015. Math Med Biol. 10.1093/imammb/dqv038. PubMed
- Liu L, et al. 2016. Nat Biotechnol. 10.1038/nbt.3461. PubMed
- Wei&Bszlig;müller S, et al. 2016. PLoS One. 11: 0149093. PubMed
- Bj?rklund &, et al. 2016. Nat Immunol. 17:451-460. PubMed
- Piekarska K, et al. 2022. Nat Commun. 13:856. PubMed
- Caduff N, et al. 2021. Cell Reports. 35(5):109056. PubMed
- Amarnath S, et al. 2010. PLoS One. 5:e1000302. PubMed
- Hunter S, et al. 2018. J Hepatol. 69:654. PubMed
- Dettling S, et al. 2018. Clin Cancer Res. 24(12). PubMed
- Engelmann C, et al. 2016. Sci Rep. 6:37926. PubMed
- Koh WH, et al. 2020. STAR Protoc. 1:100203. PubMed
- Kim ML, et al. 2021. iScience. 24:103509. PubMed
- Choe JH, et al. 2021. Sci Transl Med. 13:. PubMed
- RRID
- AB_493583 (BioLegend Cat. No. 304813) AB_493582 (BioLegend Cat. No. 304814)
- Structure
- Selectin, single chain glycoprotein, 74-95 kD
- Distribution
-
Majority of B cells, na?ve T cells, subset of memory T and NK cells, monocytes, granulocytes, thymocytes
- Function
- Leukocyte homing, leukocyte tethering, rolling
- Ligand/Receptor
- CD34, GlyCAM, MAdCAM-1
- Cell Type
- B cells, Granulocytes, Monocytes, Neutrophils, NK cells, T cells, Thymocytes, Tregs
- Biology Area
- Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
- Kishimoto T, et al. 1990. P. Natl. Acad. Sci. USA 87:2244.
- Kishimoto T, et al. 1991. Blood 78:805.
?
- Gene ID
- 6402 View all products for this Gene ID
- UniProt
- View information about CD62L on UniProt.org
